Determination of serum bile acids by glass capillary gas-liquid chromatography

1975 ◽  
Vol 64 (1) ◽  
pp. 63-68 ◽  
Author(s):  
T. Laatikainen ◽  
A. Hesso
1967 ◽  
Vol 2 (4) ◽  
pp. 365-366
Author(s):  
I. Makino ◽  
H. Taniguchi ◽  
T. Ishiya ◽  
S. Nakagawa ◽  
K. Mashimo

1965 ◽  
Vol 6 (2) ◽  
pp. 182-192 ◽  
Author(s):  
D.H. Sandberg ◽  
J. Sjövall ◽  
K. Sjövall ◽  
D.A. Turner

1966 ◽  
Vol 44 (6) ◽  
pp. 957-969 ◽  
Author(s):  
S. S. Ali ◽  
A. Kuksis ◽  
J. M. R. Beveridge

A combination of thin-layer chromatography and gas–liquid chromatography was used for the separation and determination of fecal bile acids in three middle-aged men during the last 4 days of three 8-day periods on a fat-free diet. The range of the total output was 130–650 mg/day. Lithocholic and deoxycholic acids were the major components and accounted for 65–80% of the total excretion of bile acids. Other common bile acids were present in considerably smaller quantities (0.5–11% each). In addition, evidence was obtained for the presence of a number of as yet unreported fecal bile acids. These were tentatively identified as 3α,12β-dihydroxy-, 3β,12β-dihydroxy-, 3α,7β-dihydroxy-, 3α-monohydroxy-7-keto-, 12α-monohydroxy-3-keto-, and 7β-monohydroxy-cholanic and cholanic acids. The latter three acids occurred in traces only, but the other uncommon fecal bile acids each accounted for 0.5–7% of the total excretion of bile acids. Significant variations were found in the concentrations of several of the bile acids both between different samples from the same subject and between samples from different subjects.


1984 ◽  
Vol 62 (6) ◽  
pp. 352-362 ◽  
Author(s):  
J. J. Myher ◽  
A. Kuksis

A sensitive and practical procedure has been developed for the determination of the sn-1-alk-1-enyl-2-acylglycerol moieties of natural glycerophospholipids using capillary gas–liquid chromatography with flame ionization detection. The method gives excellent resolution and quantitative estimates for molecular species according to carbon number and degree of unsaturation. The diradylglycerols are released from the glycerophospholipids by hydrolysis with phospholipase C and the alk-1-enylacylglycerols are resolved by thin-layer chromatography. Either the trimethylsilyl or the tertiary-butyldimethylsilyl ethers are then resolved at 250 °C by gas–liquid chromatography on a glass capillary column wall-coated with a polar cyanopropylsiloxane polymer using hydrogen as the carrier gas. The method has been applied to the analysis of the alk-1-enylacyl- and diacyl-glycerol moieties of the phosphatidylethanolamines of rat heart and kidney.


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