Heterogeneity and subcellular localization of hamster adipocyte α-adrenergic receptors

TMEM165 was highlighted in 2012 as the first member of the Uncharacterized Protein Family 0016 (UPF0016) related to human glycosylation diseases. Defects in TMEM165 are associated with strong Golgi glycosylation abnormalities. Our previous work has shown that TMEM165 rapidly degrades with supraphysiological manganese supplementation. In this paper, we establish a functional link between TMEM165 and SPCA1, the Golgi Ca2+/Mn2+ P-type ATPase pump. A nearly complete loss of TMEM165 was observed in SPCA1-deficient Hap1 cells. We demonstrate that TMEM165 was constitutively degraded in lysosomes in the absence of SPCA1. Complementation studies showed that TMEM165 abundance was directly dependent on SPCA1's function and more specifically its capacity to pump Mn2+ from the cytosol into the Golgi lumen. Among SPCA1 mutants that differentially impair Mn2+ and Ca2+ transport, only the Q747A mutant that favors Mn2+ pumping rescues the abundance and Golgi subcellular localization of TMEM165. Interestingly, the overexpression of SERCA2b also rescues the expression of TMEM165. Finally, this paper highlights that TMEM165 expression is linked to the function of SPCA1.

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349 Differential regulation of Kir2.x channels by alpha-1a adrenergic receptors

EP Europace ◽

10.1016/s1099-5129(05)80209-0 ◽

2005 ◽

Vol 7 ◽

pp. 71-71

Keyword(s):

Adrenergic Receptors ◽

Differential Regulation

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465 Beta-2 adrenergic receptors polymorphisms influence the effects of carvedilol in the patients with chronic heart failure

European Journal of Heart Failure Supplements ◽

10.1016/s1567-4215(07)60276-8 ◽

2007 ◽

Vol 6 (1) ◽

pp. 100-100

Author(s):

M METRA ◽

S NODARI ◽

N PEZZALI ◽

L COVOLO ◽

G ORIZIO ◽

...

Keyword(s):

Heart Failure ◽

Chronic Heart Failure ◽

Adrenergic Receptors ◽

Beta 2

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History of vascular reactivity models and their involvement in hypertension pathogenesis

VASA ◽

10.1024/0301-1526/a000659 ◽

2017 ◽

Vol 46 (6) ◽

pp. 431-439 ◽

Cited By ~ 4

Author(s):

Ana Gabriela Conceição-Vertamatti ◽

Filipy Borghi ◽

Fernando Canova ◽

Dora Maria Grassi-Kassisse

Keyword(s):

Blood Pressure ◽

Adrenergic Receptors ◽

Vascular Reactivity ◽

Multifactorial Disease ◽

Pharmacological Treatments ◽

Beta Adrenergic Receptors ◽

Vascular Area ◽

History Of ◽

Record Blood Pressure ◽

Advance Knowledge

Abstract. Hypertension is a silent and multifactorial disease. Over two centuries ago, the first device to record blood pressure was developed, making it possible to determine normotension and to establish criteria for hypertension. Since then, several studies have contributed to advance knowledge in this area, promoting significant advances in pharmacological treatments and, as a result, increasing survival of hypertensive people. The main models developed for the study of hypertension and the main findings in the vascular area are included in this review. We considered aspects related to vascular reactivity, changes in the population, and action of beta adrenergic receptors in the pathogenesis of hypertension.

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