Stimulation of lipoprotein lipase activity of rat adipose tissue and post-heparin plasma by N
                        6
-(phenylisopropyl)adenosine

The aim of this study was to determine whether the increase in lipoprotein lipase activity displayed by the adipose tissue of obese (fa/fa) rats as compared with that of lean (Fa/fa) rats could be ascribed to a change in the content or in the catalytic properties of the enzyme. The question was addressed in rats of two ages: in 7-day-old suckling and in 30-day-old post-weaning pups. Inguinal fat-pads were removed surgically (7 days of age) or after killing (30 days of age), and acetone-extract powders were prepared. The relative quantity of enzyme was assessed by immunotitration using an antiserum raised in goat against purified lipoprotein lipase from rat adipose tissue. The results indicate that increases in enzyme activity in obese animals were strictly paralleled by increases in the amount of enzyme in suckling as well as in post-weaning pups. Moreover, the apparent Km values of lipoprotein lipase for its substrate triacylglycerol were identical in the two genotypes. In conclusion, the genotype-mediated increase in lipoprotein lipase activity in adipose tissue of obese Zucker rats was fully accounted for by an increase in the content of the enzyme. In addition, this work documents the mechanism of the increase in lipoprotein lipase activity during weaning, which is mediated mainly through changes in the adipose-tissue enzyme content.

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The effect of estrogen on the lipoprotein lipase activity of rat adipose tissue.

Journal of Clinical Investigation ◽

10.1172/jci108015 ◽

1975 ◽

Vol 55 (5) ◽

pp. 1132-1135 ◽

Cited By ~ 115

Author(s):

M Hamosh ◽

P Hamosh

Keyword(s):

Adipose Tissue ◽

Lipoprotein Lipase ◽

Lipase Activity ◽

Lipoprotein Lipase Activity ◽

Rat Adipose Tissue

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The effect of nutritional state on lipoprotein lipase activity in isolated rat adipose tissue cells

Biochimica et Biophysica Acta (BBA) - Enzymology ◽

10.1016/0005-2744(67)90118-0 ◽

1967 ◽

Vol 139 (1) ◽

pp. 123-132 ◽

Cited By ~ 18

Author(s):

N. Porkrajac ◽

W.J. Lossow ◽

I.L. Chaikoff

Keyword(s):

Adipose Tissue ◽

Lipoprotein Lipase ◽

Lipase Activity ◽

Nutritional State ◽

Lipoprotein Lipase Activity ◽

Tissue Cells ◽

Rat Adipose Tissue ◽

Adipose Tissue Cells ◽

Isolated Rat

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Effects of insulin, glucocorticoids and adrenaline on the activity of rat adipose-tissue lipoprotein lipase

Biochemical Journal ◽

10.1042/bj1880185 ◽

1980 ◽

Vol 188 (1) ◽

pp. 185-192 ◽

Cited By ~ 81

Author(s):

P Ashby ◽

D S Robinson

Keyword(s):

Protein Synthesis ◽

Adipose Tissue ◽

Lipoprotein Lipase ◽

Lipase Activity ◽

Lipoprotein Lipase Activity ◽

Fat Bodies ◽

Rat Adipose Tissue ◽

Adipose Tissue Lipoprotein Lipase

The lipoprotein lipase activity of epididymal fat-bodies from starved rats was measured during incubations at 37 degrees C in vitro. Protein synthesis independent activation of the enzyme, previously observed during incubations at 25 decrease C, also occurs at 37 degrees C. Protein-synthesis-dependent increases in the activity of the enzyme occur in the presence of insulin and are markedly potentiated by glucocorticoids. The effects on the activity of the enzyme of insulin alone, or in the presence of glucocorticoids, are correlated with its effects on total protein synthesis in the tissue. Adrenaline antagonizes the increase in activity of the enzyme brought about by insulin and abolishes the potentiation of insulin action by glucocorticoids. These changes may be due, at least in part, to its stimulation of inactivation of the enzyme in the tissue. It is suggested that changes in adipose-tissue lipoprotein lipase activity that occur with changes in nutritional status in vivo result from the combined effects of changes in plasma insulin and glucocorticoid concentrations.

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