In vivo proton NMR studies of normal rat liver

The putrescine-stimulated S-adenosyl-L-methionine decarboxylases from rat liver and yeast were strongly inhibited by Berenil and to a lesser extent by Pentamidine. Ten times greater drug concentrations were needed to achieve a similar level of inhibition of a Mg2+-stimulated bacterial enzyme. The inhibition was irreversible in that extensive dialyses or precipitation with (NH4)2SO4 did not restore enzyme activity. Putrescine did not protect the enzyme against Berenil, but adenosylmethionine either alone or with putrescine partially protected the irreversible action of Berenil. The compound 4,4′-diamidinodiphenylamine, which differs from Berenil only in lacking the azo group between benzene rings, was a weaker inhibitor than Berenil, and its inhibition was reversible. Berenil also inhibited the activity of adenosylmethionine decarboxylase in vivo, by depressing the activity of the enzyme in normal rat liver, for at least 24 h after a single injection (50 mg/kg body wt.) of the drug.

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Concanavalin A receptors on normal rat liver cells, on rat liver cells in vivo transformed by diethylnitrosamine and on Zajdela ascites hepatoma cells of the rat: morphokinetic analysis of cell surface dynamics

Experimentelle Pathologie ◽

10.1016/s0014-4908(75)80088-3 ◽

1975 ◽

Vol 11 (5-6) ◽

pp. 291-304 ◽

Cited By ~ 1

Author(s):

J. Roth

Keyword(s):

Cell Surface ◽

Rat Liver ◽

Concanavalin A ◽

Liver Cells ◽

Surface Dynamics ◽

Ascites Hepatoma ◽

Rat Liver Cells ◽

Normal Rat ◽

Concanavalin A Receptors

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The regulation of ribonucleoside diphosphate reductase by the tumor promoter orotic acid in normal rat liver in vivo

Molecular Carcinogenesis ◽

10.1002/(sici)1098-2744(199903)24:3<188::aid-mc5>3.0.co;2-# ◽

1999 ◽

Vol 24 (3) ◽

pp. 188-196 ◽

Cited By ~ 3

Author(s):

Sharmila Manjeshwar ◽

Prema M. Rao ◽

Srinivasan Rajalakshmi ◽

Dittakavi S.R. Sarma

Keyword(s):

Rat Liver ◽

Orotic Acid ◽

Tumor Promoter ◽

Normal Rat ◽

Ribonucleoside Diphosphate Reductase

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Effect of Liver on Lipolysis by Normal and Postheparin Sera in the Rat

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1956.185.1.18 ◽

1956 ◽

Vol 185 (1) ◽

pp. 18-22 ◽

Cited By ~ 31

Author(s):

Judy A. Spitzer ◽

John J. Spitzer

Keyword(s):

Rat Liver ◽

Lipolytic Activity ◽

Factor Activity ◽

Rat Serum ◽

Oil Emulsion ◽

Isolated Rat Liver ◽

Normal Rat ◽

Isolated Liver ◽

Isolated Rat

Lipolysis by sera heparinized in vivo and by normal sera was studied after perfusion through isolated rat liver. The rate of clearing of an oil emulsion by serum containing clearing factor was decreased after perfusion through the liver. The extent of clearing was not always affected. Clearing factor activity was lessened by incubation with heparinase (prepared from rabbit livers). Normal rat serum exhibited consistent lipolytic activity following perfusion through the isolated liver. This activity was not due to clearing factor.

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Proton NMR Studies on the Metabolism and Biochemical Effects of Hydrazine In Vivo

Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular ◽

10.1007/978-1-4757-9424-3_53 ◽

1988 ◽

pp. 375-381 ◽

Cited By ~ 1

Author(s):

S. M. Sanins ◽

J. A. Timbrell ◽

C. Elcombe ◽

J. K. Nicholson

Keyword(s):

Proton Nmr ◽

Nmr Studies ◽

Biochemical Effects

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In vivo TESTS OF THE CONCEPT OF PHOTODYNAMIC THRESHOLD DOSE IN NORMAL RAT LIVER PHOTOSENSITIZED BY ALUMINUM CHLOROSULPHONATED PHTHALOCYANINE

Photochemistry and Photobiology ◽

10.1111/j.1751-1097.1990.tb01720.x ◽

1990 ◽

Vol 51 (3) ◽

pp. 343-349 ◽

Cited By ~ 141

Author(s):

Michael S. Patterson ◽

Brian C. Wilson ◽

Ronald Graff

Keyword(s):

Rat Liver ◽

Threshold Dose ◽

In Vivo Tests ◽

Normal Rat

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Autocrine expression of activated transforming growth factor-β1 induces apoptosis in normal rat liver

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.2001.280.1.g139 ◽

2001 ◽

Vol 280 (1) ◽

pp. G139-G148 ◽

Cited By ~ 23

Author(s):

Laura W. Schrum ◽

Mark A. Bird ◽

Olga Salcher ◽

Elmar-Reinhold Burchardt ◽

Joe W. Grisham ◽

...

Keyword(s):

Growth Factor ◽

Rat Liver ◽

Transforming Growth Factor ◽

Growth Control ◽

Rat Hepatocytes ◽

Transforming Growth Factor Β1 ◽

Hepatocyte Apoptosis ◽

Normal Rat ◽

Induced Apoptosis

The aim of this study was to determine the differential effects of latent and activated transforming growth factor (TGF)-β1in growth control of normal and proliferating hepatocytes in vivo. Rats were injected with adenoviruses expressing control transgenes (Ctrl), latent TGF-β1 [TGF-β(L)], or activated TGF-β1 [TGF-β(A)]. Additional animals underwent two-thirds partial hepatectomy (PH) 24 h after injection. Increased hepatocyte apoptosis was observed in TGF-β(A)-injected but not TGF-β(L)-injected animals 24 h postinjection (10.5%) compared with Ctrl animals (0.37%). The percent of apoptotic cells increased to 32.1% in TGF-β(A)-injected animals 48 h after injection. Furthermore, TGF-β(A)-injected rats did not survive 24 h after PH. Four hours after PH, 0.25 and 14.1% apoptotic hepatocytes were seen in Ctrl- and TGF-β(A)-injected rats, respectively. TGF-β(A)-induced apoptosis in primary rat hepatocytes was blocked with a pancaspase inhibitor. Thus autocrine expression of TGF-β(A) but not TGF-β(L) induces hepatocyte apoptosis in the normal rat liver. Rats overexpressing TGF-β(A) do not survive two-thirds PH due to hepatic apoptosis. Thus activation of TGF-β1 may be a critical step in the growth control of normal and proliferating rat hepatocytes.

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