Linkage of a Drosophila melanogaster U1 small nuclear RNA gene to the larval serum protein 1-β gene

Cajal bodies (CBs) are nuclear organelles that are usually identified by the marker protein p80-coilin. Because no orthologue of coilin is known in Drosophila melanogaster, we identified D. melanogaster CBs using probes for other components that are relatively diagnostic for CBs in vertebrate cells. U85 small CB–specific RNA, U2 small nuclear RNA, the survival of motor neurons protein, and fibrillarin occur together in a nuclear body that is closely associated with the nucleolus. Based on its similarity to CBs in other organisms, we refer to this structure as the D. melanogaster CB. Surprisingly, the D. melanogaster U7 small nuclear RNP resides in a separate nuclear body, which we call the histone locus body (HLB). The HLB is invariably colocalized with the histone gene locus. Thus, canonical CB components are distributed into at least two nuclear bodies in D. melanogaster. The identification of these nuclear bodies now permits a broad range of questions to be asked about CB structure and function in a genetically tractable organism.

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Biochemical and Immunological Studies on Larval Serum Protein 1, the Major Haemolymph Protein of Drosophila melanogaster Third-Instar Larvae

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1977.tb11782.x ◽

1977 ◽

Vol 79 (1) ◽

pp. 47-53 ◽

Cited By ~ 54

Author(s):

Jonathan WOLFE ◽

Michael E. AKAM ◽

David B. ROBERTS

Keyword(s):

Drosophila Melanogaster ◽

Serum Protein ◽

Haemolymph Protein ◽

Larval Serum Protein ◽

Third Instar Larvae

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Sequence conservation around the 5′ ends of the larval serum protein 1 genes of Drosophila melanogaster

Journal of Molecular Biology ◽

10.1016/0022-2836(86)90376-1 ◽

1986 ◽

Vol 189 (1) ◽

pp. 1-11 ◽

Cited By ~ 28

Author(s):

Stephen J. Delaney ◽

Deborah F. Smith ◽

Alan McClelland ◽

Claudio Sunkel ◽

David M. Glover

Keyword(s):

Drosophila Melanogaster ◽

Serum Protein ◽

Sequence Conservation ◽

Larval Serum Protein

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A genetic analysis of the rose-gespleten region (68C8-69B5) of Drosophila melanogaster.

Genetics ◽

10.1093/genetics/118.4.665 ◽

1988 ◽

Vol 118 (4) ◽

pp. 665-670

Author(s):

A M Hoogwerf ◽

M Akam ◽

D Roberts

Keyword(s):

Drosophila Melanogaster ◽

Genetic Analysis ◽

Serum Protein ◽

Lethal Mutations ◽

Larval Serum Protein ◽

Complementation Groups ◽

The Subject ◽

Chromosome Ii ◽

The Rose

Abstract We describe a genetic analysis of the region 68C8-69B5 defined by Df(3L)vin-7. We have induced 35 new lethal mutations in this region, which together with 20 existing lethal mutations, visible mutations, genes identified by protein products and one gene deduced from complementation data fall into 37 complementation groups in this 35-band interval. Using existing and newly induced deficiencies we have assigned these to 11 intervals defined by deficiency breakpoints. Those mutations which fell in the same breakpoint interval as the Lsp-2 gene, which codes for the abundant larval serum protein 2, were the subject of detailed study. None was rescued by the active Lsp-2 gene transformed on to chromosome II and we conclude that, as yet, we have no lethal mutations of Lsp-2.

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