Drosophila melanogaster U1 and U2 small nuclear RNA genes contain common flanking sequences

1984 ◽  
Vol 173 (4) ◽  
pp. 539-542 ◽  
Author(s):  
E. Beck ◽  
J.L. Jorcano ◽  
A. Alonso
Keyword(s):  
Drosophila Melanogaster ◽  
Small Nuclear Rna ◽  
Nuclear Rna ◽  
Flanking Sequences ◽  
Rna Genes

Varied Transcriptional Efficiencies of Multiple Arabidopsis U6 Small Nuclear RNA Genes

2007 ◽  
Vol 49 (2) ◽  
pp. 222-229 ◽  
Author(s):  
Xia Li ◽  
Dan-Hua Jiang ◽  
Kelan Yong ◽  
Da-Bing Zhang
Keyword(s):  
Small Nuclear Rna ◽  
Nuclear Rna ◽  
Rna Genes

scholarly journals Structural and functional analysis of chicken U4 small nuclear RNA genes.

1986 ◽  
Vol 6 (11) ◽  
pp. 3910-3919 ◽  
Author(s):  
M L Hoffman ◽  
G M Korf ◽  
K J McNamara ◽  
W E Stumph
Keyword(s):  
Distal Region ◽  
Xenopus Laevis Oocytes ◽  
Small Nuclear Rna ◽  
Transcriptional Enhancer ◽  
Base Pairs ◽  
Base Substitutions ◽  
Nuclear Rna ◽  
Sequence Elements ◽  
U4 Rna ◽  
Rna Genes

Two distinct chicken U4 RNA genes have been cloned and characterized. They are closely linked within 465 base pairs of each other and have the same transcriptional orientation. The downstream U4 homology is a true gene, based on the criteria that it is colinear with chicken U4B RNA and is expressed when injected into Xenopus laevis oocytes. The upstream U4 homology, however, contains seven base substitutions relative to U4B RNA. This sequence may be a nonexpressed pseudogene, but the pattern of base substitutions suggests that it more probably encodes a variant yet functional U4 RNA product not yet characterized at the RNA level. In support of this, the two U4 genes have regions of homology with each other in their 5'-flanking DNA at two positions known to be essential for the efficient expression of vertebrate U1 and U2 small nuclear RNA genes. In the case of U1 and U2 RNA genes, the more distal region (located near position-200 with respect to the RNA cap site) is known to function as a transcriptional enhancer. Although this region is highly conserved in overall structure and sequence among U1 and U2 RNA genes, it is much less conserved in the chicken U4 RNA genes reported here. Interestingly, short sequence elements present in the -200 region of the U4 RNA genes are inverted (i.e., on the complementary strand) relative to their usual orientation upstream of U1 and U2 RNA genes. Thus, the -200 region of the U4 RNA genes may represent a natural evolutionary occurrence of an enhancer sequence inversion.

Molecular characterization and chromosomal mapping of the 5S rRNA gene in Solea senegalensis: a new linkage to the U1, U2, and U5 small nuclear RNA genes

Genome ◽  
2006 ◽  
Vol 49 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Manuel Manchado ◽  
Eugenia Zuasti ◽  
Ismael Cross ◽  
Alejandro Merlo ◽  
Carlos Infante ◽  
...  
Keyword(s):  
5S Rdna ◽  
5S Rrna ◽  
Rrna Genes ◽  
Solea Senegalensis ◽  
Rrna Gene ◽  
Small Nuclear Rna ◽  
5S Rrna Gene ◽  
Nuclear Rna ◽  
Pcr Products ◽  
Rna Genes

Some units of the 5S rDNA of Solea senegalensis were amplified by PCR and sequenced. Three main PCR products (227, 441, and 2166 bp) were identified. The 227- and 441-bp fragments were characterized by highly divergent nontranscribed spacer sequences (referred to as NTS-I and NTS-II) that were 109 and 324 bp long, respectively, yet their coding sequences were nearly identical. The 2166-bp 5S rDNA unit was composed of two 5S rRNA genes separated by NTS-I and followed by a 1721-bp spacer containing the U2, U5, and U1 small nuclear RNA genes (snRNAs). They were inverted and arranged in the transcriptional direction opposite that of the 5S rRNA gene. This simultaneous linkage of 3 different snRNAs had never been observed before. The PCR products were used as probes in fluorescence in situ hybridization experiments to locate the corresponding loci on the chromosomes of S. senegalensis. A major 5S rDNA chromosomal site was located along most of the short arm of a submetacentric pair, while a minor site was detected near the centromeric region of an acrocentric pair.Key words: soleidae, pleuronectiformes, 5S rDNA, Solea, snRNAs linkage.

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