Isolation and characterization of Drosophila melanogaster U2 small nuclear RNA genes

1983 ◽  
Vol 169 (3) ◽  
pp. 691-705 ◽  
Author(s):  
A. Alonso ◽  
J.L. Jorcano ◽  
E. Beck ◽  
E. Spiess
Keyword(s):  
Drosophila Melanogaster ◽  
Small Nuclear Rna ◽  
Isolation And Characterization ◽  
Nuclear Rna ◽  
Rna Genes

scholarly journals Isolation and characterization of two linked mouse U1b small nuclear RNA genes

1983 ◽  
Vol 11 (18) ◽  
pp. 6255-6270 ◽  
Author(s):  
W.F. Marzluff ◽  
David T. Brown ◽  
Susan Lobo ◽  
Shan-Shan Wang
Keyword(s):  
Small Nuclear Rna ◽  
Isolation And Characterization ◽  
Nuclear Rna ◽  
Rna Genes

Isolation and characterization of a mitochondrial RNA polymerase from Drosophila melanogaster

1987 ◽  
Vol 65 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Michael Goldenthal ◽  
James T. Nishiura
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Nuclear Dna ◽  
Gradient Centrifugation ◽  
Mitochondrial Rna ◽  
Mitochondrial Rna Polymerase ◽  
Isolation And Characterization ◽  
Cscl Gradient ◽  
Nuclear Rna

A DNA-dependent RNA polymerase was solubilized from sucrose gradient isolated, DNase-treated mitochrondria of Drosophila melanogaster. The isolated mitochondria were not detectably contaminated with nuclear DNA as shown by CsCl gradient centrifugation and polylysine Kieselguhr chromatography. The detergent-solubilized RNA polymerase was sensitive to rifampicin, resistant to α-amanitin, had an apparent molecular mass of about 60 kilodaltons, and displayed a tendency to aggregate, both in crude extracts or when purified. The mitochondrial RNA polymerase could be distinguished from nuclear RNA polymerases on the basis of size, salt optima, rifampicin sensitivity, and α-amanitin resistance.

Isolation and characterization of three cloned fragments of human DNA coding for tRNAs and small nuclear RNA U1

Gene ◽  
1983 ◽  
Vol 22 (2-3) ◽  
pp. 211-217 ◽  
Author(s):  
R.A. Buckland ◽  
H.J. Cooke ◽  
K.L. Roy ◽  
J.E. Dahlberg ◽  
E. Lund
Keyword(s):  
Small Nuclear Rna ◽  
Dna Coding ◽  
Isolation And Characterization ◽  
Human Dna ◽  
Nuclear Rna

Varied Transcriptional Efficiencies of Multiple Arabidopsis U6 Small Nuclear RNA Genes

2007 ◽  
Vol 49 (2) ◽  
pp. 222-229 ◽  
Author(s):  
Xia Li ◽  
Dan-Hua Jiang ◽  
Kelan Yong ◽  
Da-Bing Zhang
Keyword(s):  
Small Nuclear Rna ◽  
Nuclear Rna ◽  
Rna Genes

scholarly journals Isolation and characterization of the dopa decarboxylase gene of Drosophila melanogaster

1981 ◽  
Vol 1 (6) ◽  
pp. 475-485
Author(s):  
J Hirsh ◽  
N Davidson
Keyword(s):  
Drosophila Melanogaster ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Isolation Procedure ◽  
Chromosome Band ◽  
Dopa Decarboxylase ◽  
Developmental Profile ◽  
Isolation And Characterization

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.

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