Behavior of a cross-linked attachment site: Testing the role of branch migration in site-specific recombination

1991 ◽  
Vol 220 (3) ◽  
pp. 621-629 ◽  
Author(s):  
Marlon Cowart ◽  
Stephen J. Benkovic ◽  
Howard A. Nash
Keyword(s):  
Attachment Site ◽  
Branch Migration ◽  
Site Testing ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Role of attP in Integrase-Mediated Integration of the Shigella Resistance Locus Pathogenicity Island of Shigella flexneri

2004 ◽  
Vol 48 (3) ◽  
pp. 1028-1031 ◽  
Author(s):  
Sally A. Turner ◽  
Shelley N. Luck ◽  
Harry Sakellaris ◽  
Kumar Rajakumar ◽  
Ben Adler
Keyword(s):  
Shigella Flexneri ◽  
Pathogenicity Island ◽  
Resistance Locus ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Independent Site

ABSTRACT The Shigella resistance locus (SRL) pathogenicity island (PAI) in Shigella spp. mediates resistance to streptomycin, ampicillin, chloramphenicol, and tetracycline. It can be excised from the chromosome via site-specific recombination mediated by the P4-related int gene. Here, we show that SRL PAI attP is capable of RecA-independent, site-specific, int-mediated integration into two bacterial tRNA attB sites.

The role of himA and xis in lambda site-specific recombination

1980 ◽  
Vol 138 (3) ◽  
pp. 503-512 ◽  
Author(s):  
Susan Gottesman ◽  
Ken Abremski
Keyword(s):  
Site Specific ◽  
Site Specific Recombination

Site-specific Recombination of Bacteriophage  : The Role of Host Gene Products

1979 ◽  
Vol 43 (0) ◽  
pp. 1121-1126 ◽  
Author(s):  
H. I. Miller ◽  
A. Kikuchi ◽  
H. A. Nash ◽  
R. A. Weisberg ◽  
D. I. Friedman
Keyword(s):  
Host Gene ◽  
Gene Products ◽  
Site Specific ◽  
Site Specific Recombination

Role of Gin and FIS in Site-specific Recombination

1993 ◽  
Vol 58 (0) ◽  
pp. 505-513 ◽  
Author(s):  
P. Merker ◽  
G. Muskhelishvili ◽  
A. Deufel ◽  
K. Rusch ◽  
R. Kahmann
Keyword(s):  
Site Specific ◽  
Site Specific Recombination

scholarly journals The role of theloxPspacer region in PI site-specific recombination

1986 ◽  
Vol 14 (5) ◽  
pp. 2287-2300 ◽  
Author(s):  
Ronald H. Hoess ◽  
Anna Wierzbicki ◽  
Kenneth Abremski
Keyword(s):  
Site Specific ◽  
Site Specific Recombination

scholarly journals Challenging a Paradigm: the Role of DNA Homology in Tyrosine Recombinase Reactions

2009 ◽  
Vol 73 (2) ◽  
pp. 300-309 ◽  
Author(s):  
Lara Rajeev ◽  
Karolina Malanowska ◽  
Jeffrey F. Gardner
Keyword(s):  
Dna Homology ◽  
Tyrosine Recombinase ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Conjugative Transposons ◽  
The Core ◽  
Sequence Identity ◽  
Tyrosine Recombinases ◽  
Recent Developments

SUMMARY A classical feature of the tyrosine recombinase family of proteins catalyzing site-specific recombination, as exemplified by the phage lambda integrase and the Cre and Flp recombinases, is the ability to recombine substrates sharing very limited DNA sequence identity. Decades of research have established the importance of this short stretch of identity within the core regions of the substrates. Since then, several new enzymes that challenge this paradigm have been discovered and require the role of sequence identity in site-specific recombination to be reconsidered. The integrases of the conjugative transposons such as Tn916, Tn1545, and CTnDOT recombine substrates with heterologous core sequences. The integrase of the mobilizable transposon NBU1 performs recombination more efficiently with certain core mismatches. The integration of CTX phage and capture of gene cassettes by integrons also occur by altered mechanisms. In these systems, recombination occurs between mismatched sequences by a single strand exchange. In this review, we discuss literature that led to the formulation of the current strand-swapping isomerization model for tyrosine recombinases. The review then focuses on recent developments on the recombinases that challenged the paradigm that was derived from the studies of early systems.

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