The Role of Specialized Nucleoprotein Structures in Site-specific Recombination and Initiation of DNA Replication

1984 ◽  
Vol 49 (0) ◽  
pp. 727-733 ◽  
Author(s):  
H. Echols ◽  
M. Dodson ◽  
M. Better ◽  
J.D. Roberts ◽  
R. McMacken
Keyword(s):  
Dna Replication ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Initiation Of Dna Replication

Site-specific Initiation of DNA Replication in Metazoan Chromosomes and the Role of Nuclear Organization

1993 ◽  
Vol 58 (0) ◽  
pp. 475-485 ◽  
Author(s):  
D.M. Gilbert ◽  
H. Miyazawa ◽  
F.S. Nallaseth ◽  
J.M. Ortega ◽  
J.J. Blow ◽  
...  
Keyword(s):  
Dna Replication ◽  
Nuclear Organization ◽  
Site Specific ◽  
Initiation Of Dna Replication

scholarly journals Role of Papillomavirus E1 Initiator Dimerization in DNA Replication

2005 ◽  
Vol 79 (13) ◽  
pp. 8661-8664 ◽  
Author(s):  
Stephen Schuck ◽  
Arne Stenlund
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Dna Binding Domains ◽  
Binding Domains ◽  
Severe Effect ◽  
Origin Of Dna Replication ◽  
Initiation Of Dna Replication

ABSTRACT Viral initiator proteins are polypeptides that form oligomeric complexes on the origin of DNA replication (ori). These complexes carry out a multitude of functions related to initiation of DNA replication, and although many of these functions have been characterized biochemically, little is understood about how the complexes are assembled. Here we demonstrate that loss of one particular interaction, the dimerization between E1 DNA binding domains, has a severe effect on DNA replication in vivo but has surprisingly modest effects on most individual biochemical activities in vitro. We conclude that the dimer interaction is primarily required for initial recognition of ori.

scholarly journals Role of attP in Integrase-Mediated Integration of the Shigella Resistance Locus Pathogenicity Island of Shigella flexneri

2004 ◽  
Vol 48 (3) ◽  
pp. 1028-1031 ◽  
Author(s):  
Sally A. Turner ◽  
Shelley N. Luck ◽  
Harry Sakellaris ◽  
Kumar Rajakumar ◽  
Ben Adler
Keyword(s):  
Shigella Flexneri ◽  
Pathogenicity Island ◽  
Resistance Locus ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Independent Site

ABSTRACT The Shigella resistance locus (SRL) pathogenicity island (PAI) in Shigella spp. mediates resistance to streptomycin, ampicillin, chloramphenicol, and tetracycline. It can be excised from the chromosome via site-specific recombination mediated by the P4-related int gene. Here, we show that SRL PAI attP is capable of RecA-independent, site-specific, int-mediated integration into two bacterial tRNA attB sites.

The role of himA and xis in lambda site-specific recombination

1980 ◽  
Vol 138 (3) ◽  
pp. 503-512 ◽  
Author(s):  
Susan Gottesman ◽  
Ken Abremski
Keyword(s):  
Site Specific ◽  
Site Specific Recombination

Site-specific Recombination of Bacteriophage  : The Role of Host Gene Products

1979 ◽  
Vol 43 (0) ◽  
pp. 1121-1126 ◽  
Author(s):  
H. I. Miller ◽  
A. Kikuchi ◽  
H. A. Nash ◽  
R. A. Weisberg ◽  
D. I. Friedman
Keyword(s):  
Host Gene ◽  
Gene Products ◽  
Site Specific ◽  
Site Specific Recombination

Role of Gin and FIS in Site-specific Recombination

1993 ◽  
Vol 58 (0) ◽  
pp. 505-513 ◽  
Author(s):  
P. Merker ◽  
G. Muskhelishvili ◽  
A. Deufel ◽  
K. Rusch ◽  
R. Kahmann
Keyword(s):  
Site Specific ◽  
Site Specific Recombination

Initiation of polyoma virus origin-dependent DNA replication through STAT5 activation by human granulocyte-macrophage colony-stimulating factor

Blood ◽  
2001 ◽  
Vol 97 (5) ◽  
pp. 1266-1273 ◽  
Author(s):  
Sumiko Watanabe ◽  
Rong Zeng ◽  
Yutaka Aoki ◽  
Tohru Itoh ◽  
Ken-ichi Arai
Keyword(s):  
Dna Replication ◽  
Transcriptional Activation ◽  
Tandem Repeats ◽  
T Antigen ◽  
Replication Initiation ◽  
Macrophage Colony Stimulating Factor ◽  
Initiation Of Dna Replication ◽  
Macrophage Colony ◽  
Stimulating Factor

Several lines of evidence indicate that transcriptional activation is coupled with DNA replication initiation, but the nature of initiation of DNA replication in mammalian cells is unclear. Polyoma virus replicon is an excellent system to analyze the initiation of DNA replication in murine cells because its replication requires an enhancer, and all components of replication machinery, except for DNA helicase large T antigen, are supplied by host cells. This system was used to examine the role of signal transducer and activator of transcription (STAT5) in replication initiation of polyoma replicon in the mouse lymphoid cell line BA/F3. The plasmid with tandem repeats of consensus STAT5 binding sites followed by polyoma replication origin was replicated by stimulation with human granulocyte-macrophage colony-stimulating factor (hGM-CSF) in the presence of polyoma large T antigen in BA/F3 cells. Mutation analysis of the hGM-CSF receptor β subunit revealed that only the box1 region is essential, and the C-terminal tyrosine residues are dispensable for the activity. Addition of the tyrosine kinase inhibitor genistein suppressed this replication without affecting transcriptional activation of STAT5. Because deletion analysis of STAT5 indicates the importance of the C-terminal transcriptional activation domain of STAT5 for the initiation of replication, the role of this region in the activation of replication was examined with a GAL4–STAT5 fusion protein. GAL4–STAT5 activated replication of the plasmid containing tandem repeats of GAL4 binding sites and polyoma replication origin in BA/F3 cells. Mutation analysis of GAL4–STAT5 indicated that multiple serine residues coordinately have a role in activating replication. This is the first direct evidence indicating the potential involvement of STAT5 in replication.

scholarly journals The role of DDK and Treslin-MTBP in coordinating replication licensing and pre-Initiation Complex formation

2021 ◽  
Author(s):  
Ilaria Volpi ◽  
Peter J. Gillespie ◽  
Gaganmeet Singh Chadha ◽  
J. Julian Blow
Keyword(s):  
Dna Replication ◽  
Complex Formation ◽  
S Phase ◽  
Replication Initiation ◽  
Initiation Complex ◽  
Crucial Step ◽  
Egg Extract ◽  
Initiation Of Dna Replication ◽  
Rate Limiting

AbstractTreslin/Ticrr is required for the initiation of DNA replication and binds to MTBP (Mdm2 Binding Protein). Here we show that in Xenopus egg extract, MTBP forms an elongated tetramer with Treslin containing two molecules of each protein. Immunodepletion and add-back experiments show that Treslin-MTBP is rate-limiting for replication initiation. It is recruited onto chromatin before S phase starts and recruitment continues during S phase. We show that DDK activity both increases and strengthens the interaction of Treslin-MTBP with licensed chromatin. We also show that DDK activity cooperates with CDK activity to drive the interaction of Treslin-MTBP with TopBP1 which is a regulated crucial step in pre-Initiation Complex formation. These results suggest how DDK works together with CDKs to regulate Treslin-MTBP and plays a crucial in selecting which origins will undergo initiation.

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