scholarly journals Role of attP in Integrase-Mediated Integration of the Shigella Resistance Locus Pathogenicity Island of Shigella flexneri

2004 ◽  
Vol 48 (3) ◽  
pp. 1028-1031 ◽  
Author(s):  
Sally A. Turner ◽  
Shelley N. Luck ◽  
Harry Sakellaris ◽  
Kumar Rajakumar ◽  
Ben Adler

ABSTRACT The Shigella resistance locus (SRL) pathogenicity island (PAI) in Shigella spp. mediates resistance to streptomycin, ampicillin, chloramphenicol, and tetracycline. It can be excised from the chromosome via site-specific recombination mediated by the P4-related int gene. Here, we show that SRL PAI attP is capable of RecA-independent, site-specific, int-mediated integration into two bacterial tRNA attB sites.

2004 ◽  
Vol 52 (5) ◽  
pp. 1329-1336 ◽  
Author(s):  
Harry Sakellaris ◽  
Shelley N. Luck ◽  
Keith Al-Hasani ◽  
Kumar Rajakumar ◽  
Sally A. Turner ◽  
...  

1994 ◽  
Vol 176 (20) ◽  
pp. 6414-6414
Author(s):  
Matxalen Llosa ◽  
Silvia Bolland ◽  
Guadalupe Grandoso ◽  
Fernando de la Cruz

1980 ◽  
Vol 138 (3) ◽  
pp. 503-512 ◽  
Author(s):  
Susan Gottesman ◽  
Ken Abremski

1979 ◽  
Vol 43 (0) ◽  
pp. 1121-1126 ◽  
Author(s):  
H. I. Miller ◽  
A. Kikuchi ◽  
H. A. Nash ◽  
R. A. Weisberg ◽  
D. I. Friedman

1993 ◽  
Vol 58 (0) ◽  
pp. 505-513 ◽  
Author(s):  
P. Merker ◽  
G. Muskhelishvili ◽  
A. Deufel ◽  
K. Rusch ◽  
R. Kahmann

2004 ◽  
Vol 186 (16) ◽  
pp. 5551-5554 ◽  
Author(s):  
Shelley N. Luck ◽  
Sally A. Turner ◽  
Kumar Rajakumar ◽  
Ben Adler ◽  
Harry Sakellaris

ABSTRACT Pathogenicity islands are capable of excision and insertion within bacterial chromosomes. We describe a protein, Rox, that stimulates excision of the Shigella resistance locus pathogenicity island in Shigella flexneri. Sequence analysis suggests that Rox belongs to a new subfamily of recombination directionality factors, which includes proteins from P4, enterohemorrhagic Escherichia coli, and Yersinia pestis.


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