Amino acid incorporation by nuclear membrane fraction of rat liver

Life Sciences ◽  
1976 ◽  
Vol 18 (2) ◽  
pp. 215-221 ◽  
Author(s):  
H. Ono ◽  
T. Ono ◽  
O. Wada
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Nuclear Membrane ◽  
Membrane Fraction ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

Insulin stimulus of leucine incorporation into frog liver protein

1962 ◽  
Vol 203 (4) ◽  
pp. 687-689 ◽  
Author(s):  
J. C. Penhos ◽  
M. E. Krahl
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Leucine Incorporation ◽  
Liver Protein ◽  
Amino Acid Incorporation ◽  
Insulin Effect ◽  
Acid Incorporation ◽  
Liver Slices ◽  
Stimulation Of

Slices prepared from livers of bull frogs ( Rana catesbiana), pancreatectomized and/or hypophysectomized 7 days before, were incubated 2 hr in frog Ringer-bicarbonate solution at 25 C. Incorporation of leucine-1-C14 into protein was subnormal in the pancreatectomized series. The addition of insulin in vitro, with glucose also present in the medium, produced a significant ( P < 0.01) stimulation of amino acid incorporation in the following series: livers from normal fed animals; livers from animals pancreatectomized 7 days before; and livers from animals pancreatectomized and hypophysectomized 7 days before. Neither insulin nor glucose alone gave a significant effect. These results therefore confirm and extend those obtained with rat liver slices showing that insulin can stimulate amino acid incorporation into protein when added directly to liver. The effect is relatively greatest with livers from animals pancreatectomized 7 days before; the insulin effect does not depend on the presence of the pituitary, as it is obtainable with livers from animals hypophysectomized and pancreatectomized 7 days previously.

scholarly journals Biotin-mediated protein biosynthesis

1974 ◽  
Vol 140 (3) ◽  
pp. 549-556 ◽  
Author(s):  
R. L. Boeckx ◽  
K. Dakshinamurti
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Rat Liver ◽  
Intestinal Mucosa ◽  
Protein Biosynthesis ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Stimulation Of

The effect of administration of biotin to biotin-deficient rats on protein biosynthesis was studied. Biotin treatment resulted in stimulation by more than twofold of amino acid incorporation into protein, both in vivo and in vitro in rat liver, pancreas, intestinal mucosa and skin. Analysis of the products of amino acid incorporation into liver proteins in vivo and in vitro indicated that the synthesis of some proteins was stimulated more than twofold, but others were not stimulated at all. This indicates a specificity in the stimulation of protein synthesis mediated by biotin.

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