Methods for the isolation and characterization of constituents of natural products XX. Rapid column procedures for forming isopropylidene derivatives of submicrogram to micromole amounts of diols

1976 ◽  
Vol 21 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Daniel P. Schwartz
Keyword(s):  
Natural Products ◽  
Isolation And Characterization ◽  
Isopropylidene Derivatives ◽  
Derivatives Of

scholarly journals Carbohydrate binding activities of Bradyrhizobium japonicum. II. Isolation and characterization of a galactose-specific lectin.

1990 ◽  
Vol 111 (4) ◽  
pp. 1639-1643 ◽  
Author(s):  
S C Ho ◽  
M Schindler ◽  
J L Wang
Keyword(s):  
Isoelectric Focusing ◽  
Bradyrhizobium Japonicum ◽  
Affinity Column ◽  
Carbohydrate Binding ◽  
Binding Affinities ◽  
Isolation And Characterization ◽  
Relative Binding ◽  
Mannose Derivatives ◽  
Derivatives Of

Extracts of Bradyrhizobium japonicum were fractionated on Sepharose columns covalently derivatized with lactose. Elution of the material that was specifically bound to the affinity column with lactose yielded a protein of Mr approximately 38,000. Isoelectric focusing of this sample yielded two spots with pI values of 6.4 and 6.8. This protein specifically bound to galactose-containing glycoconjugates, but did not bind either to glucose or mannose. Derivatives of galactose at the C-2 position showed much weaker binding; there was an 18-fold difference in the relative binding affinities of galactose versus N-acetyl-D-galactosamine. These results indicate that we have purified a newly identified carbohydrate-binding protein from Bradyrhizobium japonicum, that can exquisitely distinguish galactose from its derivatives at the C-2 position.

Methods for the isolation and characterization of constituents of natural products

1969 ◽  
Vol 14 (4) ◽  
pp. 556-563
Author(s):  
D.P. Schwartz ◽  
C.R. Brewington ◽  
J.L. Weihrauch ◽  
O.W. Parks
Keyword(s):  
Natural Products ◽  
Isolation And Characterization

scholarly journals Antihyperglycemic agents from Ammannia multiflora

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Harish C. Upadhyay ◽  
Natasha Jaiswal ◽  
Akhilesh K. Tamrakar ◽  
Arvind K. Srivastava ◽  
Namita Gupta ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Chromatographic Separation ◽  
Antidiabetic Drug ◽  
Antihyperglycemic Activity ◽  
Chemically Modified ◽  
Isolation And Characterization ◽  
Synthetic Derivatives ◽  
Derivatives Of

The serial chromatographic separation of chloroform and n-butanol fractions of Ammannia multiflora resulted in the isolation and characterization of 4-hydroxy-α-tetralone (1) and 3,3′-(2 R,5 R)-tetrahydrofuran-2,5-diyldiphenol (ammaniol, 2). Compound 1 was chemically modified into six semi-synthetic acyl and aryl derivatives (1A - 1F). The isolated compounds 1 and 2 along with semi-synthetic derivatives 1A - 1F were evaluated for in vitro antihyperglycemic activity employing 2-deoxyglucose uptake by L-6 rat muscle cell lines. The results indicated that both the isolates, as well as derivatives (1A - 1F), have the property to stimulate glucose uptake. Ammaniol (2) increased glucose uptake significantly (64.8%), while one of the aryl derivatives of 1, 4- O-(3,4,5-trimethoxybenzoyl)-α-tetralone (1D), showed potent antihyperglycemic activity and increased glucose uptake by 94.6%, even more than rosiglitazone (88.8%). Further, since 1D possesses better antihyperglycemic activity than rosiglitazone (standard), this might be a new safer antidiabetic drug of herbal origin.

Methods for the isolation and characterization of constituents of natural products

1968 ◽  
Vol 13 (2) ◽  
pp. 297-309 ◽  
Author(s):  
R. Bassette ◽  
C.R. Brewington ◽  
D.P. Schwartz
Keyword(s):  
Natural Products ◽  
Isolation And Characterization

Methods for the isolation and characterization of constituents of natural products

1967 ◽  
Vol 12 (2) ◽  
pp. 186-191 ◽  
Author(s):  
D.P. Schwartz ◽  
C.R. Brewington ◽  
Jennie Shamey
Keyword(s):  
Natural Products ◽  
Isolation And Characterization

Isolation and characterization of culmorin derivatives produced by Fusariumculmorum CMI 14764

1992 ◽  
Vol 70 (5) ◽  
pp. 1308-1316 ◽  
Author(s):  
Gertrude C. Kasitu ◽  
John W. ApSimon ◽  
Barbara A. Blackwell ◽  
David A. Fielder ◽  
Roy Greenhalgh ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Secondary Metabolites ◽  
Liquid Culture ◽  
Farnesyl Pyrophosphate ◽  
Isolation And Characterization ◽  
Derivatives Of

Liquid culture fermentations of Fusariumculmorum yield a wide variety of secondary metabolites. In addition to known isoprenoids, such as the trichothecenes and modified trichothecenes, four new derivatives of culmorin have been isolated and characterized by spectral analysis. These compounds are 15-hydroxyculmorone (7), and 5-hydroxy- (8), 12-hydroxy- (10), and 15-hydroxyculmorin (9). The structure of culmorone is confirmed. The biosynthesis of culmorin from farnesyl pyrophosphate is substantiated through the use of 13C-acetate and the oxidation of these derivatives is discussed.

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