Effects of X-irradiation on cell-cycle progression, induction of chromosomal aberrations and cell killing in ataxia telangiectasia (AT) fibroblasts

1985 ◽  
Vol 148 (1-2) ◽  
pp. 71-82 ◽  
Author(s):  
Hatsumi Nagasawa ◽  
Samuel A. Latt ◽  
Marc E. Lalande ◽  
John B. Little
Keyword(s):  
Cell Cycle ◽  
Chromosomal Aberrations ◽  
Ataxia Telangiectasia ◽  
Cell Cycle Progression ◽  
Cell Killing ◽  
Cycle Progression ◽  
X Irradiation

scholarly journals ATM prevents DSB formation by coordinating SSB repair and cell cycle progression

2015 ◽  
Vol 112 (13) ◽  
pp. 3997-4002 ◽  
Author(s):  
Svetlana V. Khoronenkova ◽  
Grigory L. Dianov
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Ataxia Telangiectasia ◽  
Cell Cycle Progression ◽  
Dna Double Strand Breaks ◽  
Cycle Progression ◽  
Strand Breaks ◽  
Delay Cell ◽  
Single Strand Breaks ◽  
Dna Instability

DNA single-strand breaks (SSBs) arise as a consequence of spontaneous DNA instability and are also formed as DNA repair intermediates. Their repair is critical because they otherwise terminate gene transcription and generate toxic DNA double-strand breaks (DSBs) on replication. To prevent the formation of DSBs, SSB repair must be completed before DNA replication. To accomplish this, cells should be able to detect unrepaired SSBs, and then delay cell cycle progression to allow more time for repair; however, to date there is no evidence supporting the coordination of SSB repair and replication in human cells. Here we report that ataxia-telangiectasia mutated kinase (ATM) plays a major role in restricting the replication of SSB-containing DNA and thus prevents DSB formation. We show that ATM is activated by SSBs and coordinates their repair with DNA replication. SSB-mediated ATM activation is followed by a G1 cell cycle delay that allows more time for repair and thus prevents the replication of damaged DNA and DSB accrual. These findings establish an unanticipated role for ATM in the signaling of DNA SSBs and provide important insight into the molecular defects leading to genetic instability in patients with ataxia-telangiectasia.

scholarly journals The Antitumor Enediyne C-1027 Alters Cell Cycle Progression and Induces Chromosomal Aberrations and Telomere Dysfunction

2005 ◽  
Vol 65 (12) ◽  
pp. 5344-5351 ◽  
Author(s):  
Mary M. McHugh ◽  
Loretta S. Gawron ◽  
Sei-Ichi Matsui ◽  
Terry A. Beerman
Keyword(s):  
Cell Cycle ◽  
Chromosomal Aberrations ◽  
Cell Cycle Progression ◽  
Telomere Dysfunction ◽  
Cycle Progression
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