On an important potential source of error in the routine determination of radioisotopes of low energy by liquid scintillation counting

1969 ◽  
Vol 68 (2) ◽  
pp. 355-356 ◽  
Author(s):  
P. Jordan ◽  
P. Köberle
1968 ◽  
Vol 109 (4) ◽  
pp. 645-650 ◽  
Author(s):  
E. R. B. Graham ◽  
A. Neuberger

1. The principle of radioisotope dilution was applied on a semi-micro scale to the determination of glucosamine and galactosamine in some glycoproteins, such as immunoglobulins, a urinary glycoprotein and blood-group-specific substances. 2. The glycoprotein was hydrolysed in the presence of [1−14C]glucosamine or [1−14C]galactosamine or both. The amino sugars were made to react with naphthyl isothiocyanate and the products formed were isolated by the method of Scott (1962). The specific radioactivities determined from liquid-scintillation counting and the extinction at 240mμ or 222mμ were used to calculate the content of amino sugars in the protein analysed. 3. Where the values could be compared with those found by other workers, differences were in general not very great. The advantages of the method are that high concentrations of acid can be employed and undesirable side reactions, which may occur with the free sugars, do not affect the results. A potential source of error of the method is discussed.


Fuel ◽  
2021 ◽  
Vol 291 ◽  
pp. 120084
Author(s):  
Charles G. Doll ◽  
Andrew E. Plymale ◽  
Alan Cooper ◽  
Igor Kutnyakov ◽  
Marie Swita ◽  
...  

2014 ◽  
Vol 31 (12) ◽  
pp. 2014-2021 ◽  
Author(s):  
Daniela dell’Oro ◽  
Marco Iammarino ◽  
Nicola Bortone ◽  
Michele Mangiacotti ◽  
Antonio Eugenio Chiaravalle

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