Central hemodynamic effects of recombinant human relaxin in the isolated, perfused rat heart model

1996 ◽  
Vol 87 (4) ◽  
pp. 610-612 ◽  
Author(s):  
C COULSON ◽  
J THORPJR ◽  
D MAYER ◽  
R CEFALO
Keyword(s):  
Rat Heart ◽  
Hemodynamic Effects ◽  
Heart Model ◽  
Perfused Rat Heart ◽  
Isolated Perfused Rat Heart ◽  
Central Hemodynamic

scholarly journals Use of the Isolated Perfused Heart for Evaluation of Cardiac Toxicity

1990 ◽  
Vol 18 (4a) ◽  
pp. 497-510 ◽  
Author(s):  
Peter G. Anderson ◽  
Stanley B. Digerness ◽  
Jerald L. Sklar ◽  
Paul J. Boor
Keyword(s):  
Rat Heart ◽  
Coronary Flow ◽  
Systolic Pressure ◽  
Left Ventricular ◽  
Heart Weight ◽  
Isolated Perfused Heart ◽  
Heart Model ◽  
Perfused Heart ◽  
Perfused Rat Heart ◽  
Isolated Perfused Rat Heart

The isolated perfused rat heart model can be used to evaluate cardiotoxicity, and is especially useful in distinguishing direct vs indirect cardiac injury. Various perfusion systems can be used to characterize the pathophysiologic as well as morphologic changes induced by drugs or chemicals of interest. The isolated perfused heart was used in the studies described herein to characterize the mechanism of allylamine cardiotoxicity. Rat hearts were perfused with Krebs-Henseleit buffer containing 10 mm allylamine and a latex balloon was inserted into the left ventricle to monitor pressure. Coronary flow in hearts perfused with 10 mm allylamine was similar to control hearts at 5, 10, and 30 min, but was reduced by 1 hr (11.5 ± 0.6 ml/min/g wet heart weight vs 16.0 ± 0.7, p < 0.01). Peak left ventricular systolic pressure increased in hearts perfused with allylamine for 5 min (156 ± 8 mm Hg vs 103 ± 9, p < 0.01), but by 2 hr was decreased compared to controls (89 ± 6 vs 105 ± 5, p < 0.05). End diastolic pressure was markedly increased at 2 hr (58 ± 3 vs 4 ± 0.8, p < 0.01). Morphologically, allylamine perfused hearts exhibited significant contraction band changes as well as numerous cells with marked swelling of the sarcoplasmic reticulum. The findings in this study suggest that allylamine produces direct myocardial damage that appears to be independent of coronary flow. These studies demonstrate that the isolated perfused rat heart model can be used to evaluate mechanisms of acute cardiotoxicity.

Comparative cardiotoxicity of idarubicin and doxorubicin using the isolated perfused rat heart model

1999 ◽  
Vol 10 (7) ◽  
pp. 671-676 ◽  
Author(s):  
Denis Platel ◽  
Paul Pouna ◽  
Simone Bonoron-Adèle ◽  
Jacques Robert
Keyword(s):  
Rat Heart ◽  
Heart Model ◽  
Perfused Rat Heart ◽  
Isolated Perfused Rat Heart

scholarly journals Anthrax edema toxin has cAMP-mediated stimulatory effects and high-dose lethal toxin has depressant effects in an isolated perfused rat heart model

2011 ◽  
Vol 300 (3) ◽  
pp. H1108-H1118 ◽  
Author(s):  
Caitlin W. Hicks ◽  
Yan Li ◽  
Shu Okugawa ◽  
Steven B. Solomon ◽  
Mahtab Moayeri ◽  
...  
Keyword(s):  
Rat Heart ◽  
Left Ventricular ◽  
Lethal Toxin ◽  
Heart Model ◽  
In Vivo Models ◽  
Inotropic Effects ◽  
Perfused Rat Heart ◽  
Isolated Perfused Rat Heart ◽  
Edema Toxin

While anthrax edema toxin produces pronounced tachycardia and lethal toxin depresses left ventricular (LV) ejection fraction in in vivo models, whether these changes reflect direct cardiac effects as opposed to indirect ones related to preload or afterload alterations is unclear. In the present study, the effects of edema toxin and lethal toxin were investigated in a constant pressure isolated perfused rat heart model. Compared with control hearts, edema toxin at doses comparable to or less than a dose that produced an 80% lethality rate (LD80) in vivo in rats (200, 100, and 50 ng/ml) produced rapid increases in heart rate (HR), coronary flow (CF), LV developed pressure (LVDP), dP/d tmax, and rate-pressure product (RPP) that were most pronounced and persisted with the lowest dose ( P ≤ 0.003). Edema toxin (50 ng/ml) increased effluent and myocardial cAMP levels ( P ≤ 0.002). Compared with dobutamine, edema toxin produced similar myocardial changes, but these occurred more slowly and persisted longer. Increases in HR, CF, and cAMP with edema toxin were inhibited by a monoclonal antibody blocking toxin uptake and by adefovir, which inhibits the toxin's intracellular adenyl cyclase activity ( P ≤ 0.05). Lethal toxin at an LD80 dose (50 ng/ml) had no significant effect on heart function but a much higher dose (500 ng/ml) reduced all parameters ( P ≤ 0.05). In conclusion, edema toxin produced cAMP-mediated myocardial chronotropic, inotropic, and vasodilatory effects. Vasodilation systemically with edema toxin could contribute to shock during anthrax while masking potential inotropic effects. Although lethal toxin produced myocardial depression, this only occurred at high doses, and its relevance to in vivo findings is unclear.

Warm blood cardioplegia. A study on the isolated perfused rat heart model

1992 ◽  
Vol 24 ◽  
pp. S83
Author(s):  
M. Chandra ◽  
E. Yaroslavsky-Houminer ◽  
G. Uretzky ◽  
J.B. Borman ◽  
H. Schwalb
Keyword(s):  
Rat Heart ◽  
Heart Model ◽  
Blood Cardioplegia ◽  
Perfused Rat Heart ◽  
Isolated Perfused Rat Heart ◽  
Warm Blood ◽  
Warm Blood Cardioplegia

scholarly journals Dietary red palm oil supplementation reduces myocardial infarct size in an isolated perfused rat heart model

2010 ◽  
Vol 9 (1) ◽  
pp. 64 ◽  
Author(s):  
Dirk J Bester ◽  
Krisztina Kupai ◽  
Tamas Csont ◽  
Gergu Szucs ◽  
Csaba Csonka ◽  
...  
Keyword(s):  
Infarct Size ◽  
Palm Oil ◽  
Rat Heart ◽  
Myocardial Infarct ◽  
Myocardial Infarct Size ◽  
Heart Model ◽  
Perfused Rat Heart ◽  
Red Palm Oil ◽  
Isolated Perfused Rat Heart
Sign in / Sign up

Export Citation Format

Share Document