Characterization of multiple molecular interactions between human cytomegalovirus (HCMV) and human immunodeficiency virus type 1 (HIV-1)

Virology ◽  
1990 ◽  
Vol 176 (1) ◽  
pp. 87-97 ◽  
Author(s):  
Robert F. Rando ◽  
Alagarsamy Srinivasan ◽  
Jennifer Feingold ◽  
Eva Gonczol ◽  
Stanley Plotkin
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Human Cytomegalovirus ◽  
Molecular Interactions ◽  
Immunodeficiency Virus ◽  
Hiv 1

scholarly journals Plant-Derived and Semi-Synthetic Calanolide Compounds with in Vitro Activity against Both Human Immunodeficiency Virus Type 1 and Human Cytomegalovirus

2000 ◽  
Vol 11 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Ze-Qi Xu ◽  
Earl R Kern ◽  
Louise Westbrook ◽  
Lois B Allen ◽  
Robert W Buckheit ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Double Bond ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Human Cytomegalovirus ◽  
Infected People ◽  
Immunodeficiency Virus ◽  
Hiv 1

Plant-derived and semi-synthetic calanolide compounds with anti-human immunodeficiency virus type 1 (HIV-1) activity were tested for anti-human cytomegalovirus (HCMV) activity in both cytopathic effect inhibition and plaque reduction assays. The results indicated that the anti-HCMV activity of calanolide compounds does not correlate with their activity against HIV-1. The semi-synthetic 12-keto derivatives tended to be more active against HCMV than the corresponding 12-OH congeners, which were more active against HIV-1. It appeared that the 7,8-unsaturated double bond in the chromene ring played a certain role in maintaining activities against both HCMV and HIV-1. Saturation of the double bond increased the EC50 values against both viruses, with concomitant increase in toxicity. The calanolide compounds reported here are the first non-nucleoside analogues capable of inhibiting both HIV-1 and HCMV and, therefore, may be useful chemoprophylactic agents for HCMV in HIV-infected people or vice versa.

scholarly journals Identification and Characterization of a Peptide That Specifically Binds the Human, Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody b12

2001 ◽  
Vol 75 (14) ◽  
pp. 6692-6699 ◽  
Author(s):  
Michael B. Zwick ◽  
Lori L. C. Bonnycastle ◽  
Alfredo Menendez ◽  
Melita B. Irving ◽  
Carlos F. Barbas ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Binding Site ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Human Monoclonal Antibody ◽  
Recombinant Polypeptide ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT Human monoclonal antibody (MAb) b12 recognizes a conformational epitope that overlaps the CD-4-binding site of the human immunodeficiency virus type 1 (HIV-1) envelope. MAb b12 neutralizes a broad range of HIV-1 primary isolates and protects against primary virus challenge in animal models. We report here the discovery and characterization of B2.1, a peptide that binds specifically to MAb b12. B2.1 was selected from a phage-displayed peptide library by using immunoglobulin G1 b12 as the selecting agent. The peptide is a homodimer whose activity depends on an intact disulfide bridge joining its polypeptide chains. Competition studies with gp120 indicate that B2.1 occupies the b12 antigen-binding site. The affinity of b12 for B2.1 depends on the form in which the peptide is presented; b12 binds best to the homodimer as a recombinant polypeptide fused to the phage coat. Originally, b12 was isolated from a phage-displayed Fab library constructed from the bone marrow of an HIV-1-infected donor. The B2.1 peptide is highly specific for b12 since it selected only phage bearing b12 Fab from this large and diverse antibody library.

Characterization of human immunodeficiency virus type-1 from HIV-1 seropositive cases with undetectable viremia

2004 ◽  
Vol 30 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Chunfu Yang ◽  
Ming Li ◽  
Faye Cowart ◽  
Donna Rudolph ◽  
Bharat Parekh ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunodeficiency Virus ◽  
Undetectable Viremia ◽  
Hiv 1

Molecular Interactions between Human Immunodeficiency Virus Type 1 and Human Cytomegalovirus

1990 ◽  
Vol 616 (1 AIDS) ◽  
pp. 54-63 ◽  
Author(s):  
PETER A. BARRY ◽  
ELISSA PRATT-LOWE ◽  
DONALD J. ALCENDOR ◽  
RONALD E. UNGER ◽  
PAUL A. LUCIW
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Human Cytomegalovirus ◽  
Molecular Interactions ◽  
Immunodeficiency Virus
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