A method for the identification of sulfated glycopeptide by two-dimensional electrophoresis on cellulose acetate membrane

1986 ◽  
Vol 12 (4) ◽  
pp. 239-246 ◽  
Author(s):  
Makoto Yokoyama ◽  
Haruhiko Tanaka ◽  
Syuichi Yoshihara ◽  
Masahiko Endo
Keyword(s):  
Cellulose Acetate ◽  
Cellulose Acetate Membrane ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Direct quantitation of glycosaminoglycans in 2 mL of urine from patients with mucopolysaccharidoses.

1981 ◽  
Vol 27 (1) ◽  
pp. 124-128 ◽  
Author(s):  
R W Burlingame ◽  
G H Thomas ◽  
R L Stevens ◽  
K Schmid ◽  
H W Moser
Keyword(s):  
Cellulose Acetate ◽  
Alcian Blue ◽  
Keratan Sulfate ◽  
Two Dimensional ◽  
Chondroitinase Abc ◽  
Two Dimensional Electrophoresis ◽  
Specific Degradation ◽  
Dimensional Electrophoresis

Abstract Glycosaminoglycans in urine from patients representing the major different mucopolysaccharidoses were separated and measured by use of a procedure that requires only 2 mL of urine. The compounds were resolved by two-dimensional electrophoresis on cellulose acetate plates and made visible by staining with Alcian Blue. They were identified by co-migration with standard glycosaminoglycans, by digestion with specific glycosidases, and by specific degradation with HNO2. They were quantitated by comparing the absorbance of eluates of the stained spots to appropriate standard curves for each glycosaminoglycan. This study revealed additional findings. About half of the patients excreted small amounts of heparin. Further, the keratan sulfate in samples from Morquio's disease patients migrated differently from authentic keratan sulfate unless digested with chondroitinase ABC. Our results for these diseases are in harmony with earlier reports.

scholarly journals Two-dimensional electrophoresis of native proteins on cellulose acetate membranes.

1989 ◽  
Vol 33 (4) ◽  
pp. 177-180
Author(s):  
Mochihiko Ohashi ◽  
Tosifusa Toda ◽  
Toshiko Fujita ◽  
Mitsutaka Yoshida
Keyword(s):  
Cellulose Acetate ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Native Proteins ◽  
Cellulose Acetate Membranes ◽  
Dimensional Electrophoresis
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