Spontaneous and cyclophosphamide-induced sister-chromatid exchanges in diploid and endoreduplicated tetraploid metaphases of preimplantation mouse embryos

1987 ◽  
Vol 192 (2) ◽  
pp. 137-140 ◽  
Author(s):  
Richard Vogel ◽  
Horst Spielmann
1982 ◽  
Vol 103 (3-6) ◽  
pp. 315-320 ◽  
Author(s):  
William W. Au ◽  
Nancy Obergoenner ◽  
Karen L. Goldenthal ◽  
Peter M. Corry ◽  
Veronica Willingham

Author(s):  
D. G. Chase ◽  
W. Winters ◽  
L. Piko

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.


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