The effect of cessation of growth on protein synthesis in a mutant of Chinese hamster ovary cells with a temperature-sensitive leucyl-tRNA synthetase

1982 ◽  
Vol 696 (1) ◽  
pp. 94-101
Author(s):  
James S. Hutchison ◽  
Kivie Moldave
Keyword(s):  
Protein Synthesis ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Trna Synthetase ◽  
Temperature Sensitive ◽  
Ovary Cells

scholarly journals Isolation of Chinese hamster ovary cells that overproduce asparaginyl-tRNA synthetase.

1984 ◽  
Vol 4 (9) ◽  
pp. 1939-1941
Author(s):  
R E Cirullo ◽  
J J Wasmuth
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Trna Synthetase ◽  
Temperature Sensitive ◽  
Ovary Cells ◽  
Resistant Phenotype ◽  
Lines Of Evidence

Temperature-resistant revertants, derived from the temperature-sensitive CHO asparaginyl-tRNA synthetase mutant, Asn-5, were isolated and characterized. Several lines of evidence indicate that the temperature-resistant phenotype of the revertants is due to their overproducing the same altered enzyme present in the Asn-5 parent.

Isolation of Chinese hamster ovary cells that overproduce asparaginyl-tRNA synthetase

1984 ◽  
Vol 4 (9) ◽  
pp. 1939-1941
Author(s):  
R E Cirullo ◽  
J J Wasmuth
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Trna Synthetase ◽  
Temperature Sensitive ◽  
Ovary Cells ◽  
Resistant Phenotype ◽  
Lines Of Evidence

Temperature-resistant revertants, derived from the temperature-sensitive CHO asparaginyl-tRNA synthetase mutant, Asn-5, were isolated and characterized. Several lines of evidence indicate that the temperature-resistant phenotype of the revertants is due to their overproducing the same altered enzyme present in the Asn-5 parent.

Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines

1989 ◽  
Vol 9 (5) ◽  
pp. 1832-1838
Author(s):  
K J Kontis ◽  
S M Arfin
Keyword(s):  
Cdna Clone ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Mrna Translation ◽  
Resistant Cell ◽  
Trna Synthetase ◽  
Expression Library ◽  
Hybridization Probe ◽  
Ovary Cells

A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA lambda gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.

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