scholarly journals Biochemical characterization of a mutant asparaginyl-tRNA synthetase from Chinese hamster ovary cells.

1978 ◽  
Vol 253 (1) ◽  
pp. 58-62 ◽  
Author(s):  
I.L. Andrulis ◽  
C.S. Chiang ◽  
S.M. Arfin ◽  
T.A. Miner ◽  
G.W. Hatfield
Keyword(s):  
Chinese Hamster Ovary ◽  
Biochemical Characterization ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Trna Synthetase ◽  
Ovary Cells

Biochemical characterization of theO-glycans on recombinant glycophorin A expressed in Chinese hamster ovary cells

1994 ◽  
Vol 11 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Peter P�hlsson ◽  
Douglas P. Blackall ◽  
Maciej Ugorski ◽  
Marcin Czerwinski ◽  
Steven L. Spitalnik
Keyword(s):  
Chinese Hamster Ovary ◽  
Biochemical Characterization ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Glycophorin A ◽  
Ovary Cells

scholarly journals Cloning and characterization of small circular DNA from Chinese hamster ovary cells.

1984 ◽  
Vol 4 (1) ◽  
pp. 173-180 ◽  
Author(s):  
S W Stanfield ◽  
D R Helinski
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Single Copy ◽  
Chromosomal Dna ◽  
Cho Cell ◽  
Ovary Cells ◽  
Chromosomal Sequences

Small polydisperse circular (spc) DNA was isolated and cloned, using BglII from Chinese hamster ovary (CHO) cells. The properties of 47 clones containing at least 43 different BglII fragments are reported. The majority of the clones probably contain entire sequences from individual spcDNA molecules. Most of the clones were homologous to sequences in CHO cell chromosomal DNA, and many were also homologous to mouse LMTK- cell chromosomal sequences. The majority of homologous CHO cell chromosomal sequences were repetitive, although a few may be single copy. Only a small fraction of cloned spcDNA molecules were present in every cell; most occurred less frequently than once in 15 cells. Localization studies indicated that at least a portion of spcDNA is associated with the nucleus in CHO cells.

Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines

1989 ◽  
Vol 9 (5) ◽  
pp. 1832-1838
Author(s):  
K J Kontis ◽  
S M Arfin
Keyword(s):  
Cdna Clone ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Mrna Translation ◽  
Resistant Cell ◽  
Trna Synthetase ◽  
Expression Library ◽  
Hybridization Probe ◽  
Ovary Cells

A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA lambda gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.

scholarly journals Isolation of Chinese hamster ovary cells that overproduce asparaginyl-tRNA synthetase.

1984 ◽  
Vol 4 (9) ◽  
pp. 1939-1941
Author(s):  
R E Cirullo ◽  
J J Wasmuth
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Trna Synthetase ◽  
Temperature Sensitive ◽  
Ovary Cells ◽  
Resistant Phenotype ◽  
Lines Of Evidence

Temperature-resistant revertants, derived from the temperature-sensitive CHO asparaginyl-tRNA synthetase mutant, Asn-5, were isolated and characterized. Several lines of evidence indicate that the temperature-resistant phenotype of the revertants is due to their overproducing the same altered enzyme present in the Asn-5 parent.

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