Structural analysis of an outer surface protein from the Lyme disease spirochete, Borrelia burgdorferi, using circular dichroism and fluorescence spectroscopy

1992 ◽  
Vol 1120 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Louisa Lee France ◽  
Jan Kieleczawa ◽  
John J. Dunn ◽  
Geoffrey Hínd ◽  
John C. Sutherland
Keyword(s):  
Circular Dichroism ◽  
Structural Analysis ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Fluorescence Spectroscopy ◽  
Outer Surface ◽  
Surface Protein ◽  
Outer Surface Protein ◽  
Circular Dichroïsm

A new specific serodiagnosis system for Lyme disease: use of synthetic peptides derived from outer surface protein C of Borrelia burgdorferi

1997 ◽  
Vol 9 (1) ◽  
pp. 21-25 ◽  
Author(s):  
M. Ikushima ◽  
S. Kawahashi ◽  
Y. Ohzeki ◽  
Y. Okuyama ◽  
E. Isogai ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Synthetic Peptides ◽  
Protein C ◽  
Surface Protein ◽  
Outer Surface Protein

Immunogenicity of a recombinant Borrelia burgdorferi outer surface protein A vaccine against Lyme disease in children

1999 ◽  
Vol 135 (5) ◽  
pp. 575-579 ◽  
Author(s):  
Henry M. Feder ◽  
Jeri Beran ◽  
Christian Van Hoecke ◽  
Betsy Abraham ◽  
Norbert De Clercq ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Protein A ◽  
Surface Protein ◽  
Outer Surface Protein A ◽  
Outer Surface Protein

scholarly journals Antibody response to OspC-I synthetic peptide derived from outer surface protein C of Borrelia burgdorferi in sera from Japanese forestry workers

1999 ◽  
Vol 122 (3) ◽  
pp. 429-433 ◽  
Author(s):  
M. IKUSHIMA ◽  
F. YAMADA ◽  
S. KAWAHASHI ◽  
Y. OKUYAMA ◽  
K. MATSUI
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Synthetic Peptide ◽  
Protein C ◽  
Surface Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Forestry Workers ◽  
Outer Surface Protein

The prevalence of antibodies against Lyme disease spirochaetes in serum samples from 80 forestry workers at high occupational risk of Lyme disease was surveyed by enzyme-linked immunosorbent assay (ELISA) with the OspC-I synthetic peptide. The peptide is part of the outer surface protein C (OspC) amino acid sequence located in the region conserved among Borrelia burgdorferi sensu stricto or sensu lato. Positivity for antibodies against OspC-I was observed in 25 (31·3%) of the forestry workers. Of these positive cases, 12 (15·0%) and 19 (23·8%) were positive for immunoglobulin M (IgM) and IgG antibody, respectively. Among 62 workers who were negative for IgG antibody against B. garinii or B. japonica in our previous study, 9 (14·5%) and 4 (6·5%) were positive for IgM and IgG antibody, respectively, in OspC-I ELISA. These results demonstrate for the first time that Lyme disease in forestry workers can be revealed using OspC-I ELISA. We conclude that forestry workers who show positive results for antibodies against OspC-I have very likely been exposed to Lyme disease spirochaetes, and that those who show positivity for IgM antibody against OspC-I may be in the early stage of Lyme disease.

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