Suspension cultures of Lotus corniculatus L., cv. Leo, were treated with the herbicides 2,4-D (2,4-dichlorophenoxyacetic acid) (10–20 ppm) or chlorsulfuron (2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl]-benzenesulfonamide) (0.5–1.0 ppb) to select for either herbicide resistance or tolerance. Effects of the selection process on regeneration, pollen, chromosome number, and herbicide sensitivity of regenerates were evaluated. Plantlets regenerated from selective 2,4-D cultures exhibited low in vitro rooting percentage, a high percentage of shrivelled pollen, and variation in chromosome number. The effect of 2,4-D was confounded, in some cases, with the effect of duration of culture period. A slight degree of tolerance was observed in one of the selections to chlorsulfuron at 10 to 20 g ha−1. No sign of tolerance to 2,4-D was observed at 1.0 kg ha−1.
In vitro selection for a OneMax DNA
evolutionary computation
