Hepatitis A virus antigen comprising inactivated or attenuated complete particles containing RNA and/or immature empty particles, isolated from tissue culture for use as vaccine

Vaccine ◽  
1990 ◽  
Vol 8 (3) ◽  
pp. 292
Keyword(s):  
Tissue Culture ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

scholarly journals Quantification of Hepatitis A Virus in Shellfish by Competitive Reverse Transcription-PCR with Coextraction of Standard RNA

1999 ◽  
Vol 65 (1) ◽  
pp. 322-326 ◽  
Author(s):  
Charlotte Arnal ◽  
Virginie Ferre-Aubineau ◽  
Berangere Mignotte ◽  
Berthe Marie Imbert-Marcille ◽  
Sylviane Billaudel
Keyword(s):  
Tissue Culture ◽  
Reverse Transcription ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Internal Standard ◽  
Wild Type ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Reproducible Method ◽  
Dna Enzyme Immunoassay

ABSTRACT To quantify hepatitis A virus (HAV) in experimentally contaminated mussels, we developed an internal standard RNA with a 7-nucleotide deletion for competitive reverse transcription (RT)-PCR. Deposited directly into the sample, this standard was used both as extraction control and as quantification tool. After coextraction and competitive RT-PCR, standard and wild-type products were detected by differential hybridization with specific probes and a DNA enzyme immunoassay. The quantifiable range with this reproducible method was 104 to 107 copies of HAV/gram or 400 to 106 50% tissue culture infective doses/ml.

scholarly journals Immunofluorescence of hepatitis A virus antigen in chimpanzees.

1978 ◽  
Vol 21 (2) ◽  
pp. 663-665 ◽  
Author(s):  
B L Murphy ◽  
J E Maynard ◽  
D W Bradley ◽  
J W Ebert ◽  
L R Mathiesen ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

Relapsing hepatitis in a child, associated with isolation of hepatitis A virus antigen from the liver

1988 ◽  
Vol 147 (3) ◽  
pp. 333-333 ◽  
Author(s):  
J. N. van den Anker ◽  
R. N. Sukhai ◽  
A. M. Dumas
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

Expression of a hepatitis A virus antigen in Lactococcus lactis and Escherichia coli and evaluation of its immunogenicity

2013 ◽  
Vol 97 (10) ◽  
pp. 4333-4342 ◽  
Author(s):  
Aleš Berlec ◽  
Tadej Malovrh ◽  
Petra Zadravec ◽  
Andrej Steyer ◽  
Matjaž Ravnikar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactococcus Lactis ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Antigen

Inactivation of Hepatitis A Virus and a Calicivirus by High Hydrostatic Pressure†

2002 ◽  
Vol 65 (10) ◽  
pp. 1605-1609 ◽  
Author(s):  
DAVID H. KINGSLEY ◽  
DALLAS G. HOOVER ◽  
EFI PAPAFRAGKOU ◽  
GARY P. RICHARDS
Keyword(s):  
Tissue Culture ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Inactivation ◽  
Dependent Manner ◽  
Norwalk Virus ◽  
Rnase Protection ◽  
Infectious Dose

Potential application of high hydrostatic pressure processing (HPP) as a method for virus inactivation was evaluated. A 7-log10 PFU/ml hepatitis A virus (HAV) stock, in tissue culture medium, was reduced to nondetectable levels after exposure to more than 450 MPa of pressure for 5 min. Titers of HAV were reduced in a time- and pressure-dependent manner between 300 and 450 MPa. In contrast, poliovirus titer was unaffected by a 5-min treatment at 600 MPa. Dilution of HAV in seawater increased the pressure resistance of HAV, suggesting a protective effect of salts on virus inactivation. RNase protection experiments indicated that viral capsids may remain intact during pressure treatment, suggesting that inactivation was due to subtle alterations of viral capsid proteins. A 7-log10 tissue culture infectious dose for 50% of the cultures per ml of feline calicivirus, a Norwalk virus surrogate, was completely inactivated after 5-min treatments with 275 MPa or more. These data show that HAV and a Norwalk virus surrogate can be inactivated by HPP and suggest that HPP may be capable of rendering potentially contaminated raw shellfish free of infectious viruses.

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