virus inactivation
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Author(s):  
Hasin Feroz ◽  
Naresh Chennamsetty ◽  
Sara Byers ◽  
Melissa Holstein ◽  
Zheng Jian Li ◽  
...  

2021 ◽  
pp. 2100320
Author(s):  
Pratik U. Joshi ◽  
Christa L. Meingast ◽  
Xuankuo Xu ◽  
Melissa Holstein ◽  
Hasin Feroz ◽  
...  
Keyword(s):  
Low Ph ◽  

2021 ◽  
pp. 100191
Author(s):  
Sergio Rius-Rocabert ◽  
Javier Arranz-Herrero ◽  
Adolfo Fernández-Valdés ◽  
Marzia Marciello ◽  
Sandra Moreno ◽  
...  

2021 ◽  
Vol 34 ◽  
pp. 100716
Author(s):  
Ilaria De Pasquale ◽  
Chiara Lo Porto ◽  
Massimo Dell’Edera ◽  
Maria Lucia Curri ◽  
Roberto Comparelli
Keyword(s):  

Author(s):  
Hasin Feroz ◽  
Naresh Chennamsetty ◽  
Sara Byers ◽  
Melissa Holstein ◽  
Sanchayita Ghose

Detergent-mediated virus inactivation (VI) provides a valuable orthogonal strategy for viral clearance particularly for next generation continuous manufacturing. Furthermore, there exists an industry-wide need to replace the conventionally employed detergent, Triton X-100, with eco-friendly alternatives. This study provides a systematic approach to screen detergents as VI agents through the study of VI of three different enveloped viruses for monoclonal antibodies and fusion proteins. We investigated three major aspects of VI namely, the impact of VI agent on the therapeutic quality attributes, clearance of the VI agent and other impurities through subsequent chromatographic steps and lastly the efficacy of VI for the said detergent. Several quality attributes such as charge variance, oxidation, deamidation, glycosylation and aggregation were investigated. Aggregation was a key indicator of stability. Experimental and modeling data was used to decipher the mechanism and kinetics of aggregation for pH sensitive molecules by exploring worst case VI conditions. We found product aggregation and its kinetics to be driven by extrinsic factors such as detergent and protein concentration. Aggregation was also impacted by initial aggregation level as well as intrinsic factors such as the protein sequence and detergent hydrophobicity and critical micelle concentration (CMC). VI efficiency was dependent on the virus tested, duration of incubation as well as detergent CMC and concentration. Dodecyl maltopyranoside (DDM) was found to be a promising candidate for potential application in VI. Knowledge gained here on factors driving product stability and VI provides valuable insight to design, standardize and optimize conditions (concentration, duration of inactivation) for screening of detergent-mediated VI.


2021 ◽  
Vol 9 (10) ◽  
pp. 2096
Author(s):  
Takashi Yokoyama ◽  
Tomoyasu Nishimura ◽  
Yoshifumi Uwamino ◽  
Kenjiro Kosaki ◽  
Koichi Furusaki ◽  
...  

Here, the virucidal effect of calcium bicarbonate with a mesoscopic structure (CAC-717) on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was determined. Assays showed that CAC-717 had a strong virucidal effect on all examined SARS-CoV-2 isolates, including variant strains. The viral infectivity decreased within 15 s, and the virucidal activity of CAC-717 at a 1:49 ratio was similar to that of ethanol disinfectant. CAC-717 neutralization eliminated this virucidal effect, indicating that the alkaline condition of CAC-717 is important for virus inactivation and is an indicator of its mesoscopic structure and virucidal activity. The virucidal effect was observed in the presence of organic matter (bovine serum albumin). CAC-717 is a non-invasive and non-flammable substance with a low environmental burden, and its usefulness as a novel disinfectant has been confirmed.


Author(s):  
A. Schulz ◽  
K. Methling ◽  
M. Lalk ◽  
A. Eisenbarth ◽  
M. Keller ◽  
...  

AbstractIxodid ticks represent vectors and reservoirs for a broad range of zoonotic pathogens. Collected ticks from field studies are therefore usually stored in ethanol, which in higher concentrations effectively inactivates most of the known tick-borne pathogens. Although commonly practiced as gold standard for inactivation, hardly any scientific data demonstrate that ethanol sufficiently penetrates the comparatively thick cuticula of ticks. Therefore, Amblyomma hebraeum tick pools were stored for 21 days in ethanol (96%). Afterwards, the ethanol was removed and the ticks were homogenized. Quantitative 1H-NMR spectroscopic analysis was applied to determine the residual concentration of ethanol inside the ticks. 1H-NMR spectroscopic analysis revealed that ethanol constituted 28.3–42.6 mg of the total weight of three ticks in the pools (89.9–121.5 mg). In addition, the low-pathogenic Hazara orthonairovirus (HAZV) was used as a cell culture model for this study. The virus was exposed to ethanol concentrations between 0 and 60% and incubated under various temperature conditions for four time periods. Afterwards, the residual virus infectivity was determined by titration. Following ethanol exposure, HAZV did not grow in cells after 9 h of exposure to an ethanol concentration of 25%. These results demonstrate an extremely low ethanol resistance of the virus, which was generally in line with previously reported ethanol inactivation data for Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV). After prolonged storage and impregnation, comparable ethanol concentrations are achieved in the ticks, indicating the suitability of this inactivation method also for Bunyaviruses in ticks. At the very least, a massive virus inactivation can be assumed. Definitive proof of virus inactivation would require a bioassay of ethanol-treated infected ticks under appropriate biosafety conditions.


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