Amphibian acid phosphatase—III. Electrophoretic analysis of tissue and plasma proteins and acid phosphatase isozymes in Rana pipiens

Author(s):  
J.A. Piechowski ◽  
D.J. McWhinnie
1945 ◽  
Vol 60 (1) ◽  
pp. 14-17 ◽  
Author(s):  
B. F. Chow ◽  
J. B. Allison ◽  
W. H. Cole ◽  
R. D. Seeley

1949 ◽  
Vol 217 (2) ◽  
pp. 206-214 ◽  
Author(s):  
Henry A. Rafsky ◽  
Bernard Newman ◽  
Charles I. Krieger

1985 ◽  
Vol 249 (3) ◽  
pp. R290-R295
Author(s):  
D. R. Robertson

Ionized calcium [Ca2+] in the amphibian Rana pipiens was measured over 18 mo. An annual variation about the mean of 1.10 mmol/l was observed that peaked in April (1.36 mmol/l) and attained minimal levels in October (0.84 mmol/l). Plasma total calcium [Ca]t, plasma proteins, and the bound calcium fraction also exhibited annual oscillations. The annual mean binding capacity of plasma proteins for calcium was established at 0.0175 mmol/g. Of the plasma total calcium in spring animals, nonprotein-bound calcium complexes [Ca]c were less than 3% of the total whereas 80% was composed of [Ca2+] and 17% as protein-bound calcium [Ca]pb. In autumn [Ca2+] composed 40%, [Ca]pb 30%, and [Ca]c 30% of plasma total calcium. An equilibrium exists between [Ca2+] and [Ca]c, which maintains a combined annual mean of 1.48 mmol/l calcium. Diurnal variations superimposed on the annual cycles are modulated by apparent movement of plasma water and an intrinsic 12-h rhythm for [Ca2+]. No definite associations could be made with the entrainment of specific rhythms of the three calcium fractions with the light-dark cycle.


1975 ◽  
Vol 66 (4) ◽  
pp. 237-241 ◽  
Author(s):  
CHARLES T. PECK ◽  
CHARLES J. BIGGERS

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