Hydrogen production by marine photosynthetic bacteria: Effect of environmental factors and substrate specificity on the growth of a hydrogen-producing marine photosynthetic bacterium, Chromatium sp. Miami PBS 1071

ABSTRACT A major route for hydrogen production by purple photosynthetic bacteria is biological nitrogen fixation. Nitrogenases reduce atmospheric nitrogen to ammonia with the concomitant obligate production of molecular hydrogen. However, hydrogen production in the context of nitrogen fixation is a rather inefficient process because about 75% of the reductant consumed by the nitrogenase is used to generate ammonia. In this study we describe a selection strategy to isolate strains of purple photosynthetic bacteria in which hydrogen production is necessary for growth and independent of nitrogen fixation. We obtained four mutant strains of the photosynthetic bacterium Rhodopseudomonas palustris that produce hydrogen constitutively, even in the presence of ammonium, a condition where wild-type cells do not accumulate detectable amounts of hydrogen. Some of these strains produced up to five times more hydrogen than did wild-type cells growing under nitrogen-fixing conditions. Transcriptome analyses of the hydrogen-producing mutant strains revealed that in addition to the nitrogenase genes, 18 other genes are potentially required to produce hydrogen. The mutations that caused constitutive hydrogen production mapped to four different sites in the NifA transcriptional regulator in the four different strains. The strategy presented here can be applied to the large number of diverse species of anoxygenic photosynthetic bacteria that are known to exist in nature to identify strains for which there are fitness incentives to produce hydrogen.

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Photofermentative hydrogen production by immobilized photosynthetic bacteria: Current perspectives and challenges

Renewable and Sustainable Energy Reviews ◽

10.1016/j.rser.2021.110796 ◽

2021 ◽

Vol 141 ◽

pp. 110796

Author(s):

Emrah Sagir ◽

Siamak Alipour

Keyword(s):

Hydrogen Production ◽

Photosynthetic Bacteria

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Hydrogen production by non-photosynthetic bacteria

International Journal of Hydrogen Energy ◽

10.1016/0360-3199(85)90092-8 ◽

1985 ◽

Vol 10 (4) ◽

pp. 227-231 ◽

Cited By ~ 6

Author(s):

S HUANG ◽

C SECOR ◽

R ASCIONE ◽

R ZWEIG

Keyword(s):

Hydrogen Production ◽

Photosynthetic Bacteria

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Efficient hydrogen production using a multi-layered photobioreactor and a photosynthetic bacterium mutant with reduced pigment

International Journal of Hydrogen Energy ◽

10.1016/j.ijhydene.2006.06.019 ◽

2006 ◽

Vol 31 (11) ◽

pp. 1522-1526 ◽

Cited By ~ 48

Author(s):

T KONDO ◽

T WAKAYAMA ◽

J MIYAKE

Keyword(s):

Hydrogen Production ◽

Photosynthetic Bacterium

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Effects of algae, yeast and photosynthetic bacteria diets on survival and growth performance in the fairy shrimp, Streptocephalus sirindhornae (Branchiopoda, Anostraca)

Crustaceana ◽

10.1163/15685403-00003847 ◽

2018 ◽

Vol 91 (12) ◽

pp. 1505-1522 ◽

Cited By ~ 3

Author(s):

Chewapat Saejung ◽

Anuwat Chaiyarat ◽

La-Orsri Sanoamuang

Keyword(s):

Growth Performance ◽

Photosynthetic Bacteria ◽

Photosynthetic Bacterium ◽

Ammonia Concentration ◽

Fairy Shrimp ◽

Shrimp Culture ◽

Survival And Growth ◽

Nitrate And Nitrite ◽

First Time

Abstract This study investigated the effects on survival, growth performance and water quality of algae, yeast and photosynthetic bacteria as diets for larval and immature/mature Streptocephalus sirindhornae. Survival of the larvae fed with the alga Chlorella vulgaris and the photosynthetic bacterium Rhodopseudomonas faecalis for 5 days was higher than 80%, while those fed with the yeast Saccharomyces cerevisiae was 4.4%. After feeding the 6-day-old fairy shrimp until they reached 30 days of age, those fed with bacteria showed the highest survival and growth rate. Ammonia, nitrate and nitrite concentrations in the water treated with R. faecalis were the lowest. The highest ammonia concentration, the lowest dissolved oxygen, and excessive turbidity were found in the yeast treatment. Frequent water replacement could not overcome this, which resulted in the low survival rate. Utilization of photosynthetic bacteria in fairy shrimp culture is for the first time reported herein, and the disadvantages of using yeast in S. sirindhornae cultivation are discussed.

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