A comparative study of the complement fixation test for mycoplasma hyopneumoniae in SPF and non-SPF herds of swine in Illinois

Test antigens prepared respectively from adult and from larval Trichina were compared by means of the complement fixation test on serum from rabbits infected experimentally with varying numbers of Trichinae. While significant levels of reactivity with “adult” antigen were shown to occur, it had no apparent advantage over the “larval” antigen in the early detection of antibodies.

Download Full-text

Evaluation of the Complement Fixation Test in Amebiasis

Gastroenterology ◽

10.1016/s0016-5085(52)80038-1 ◽

1952 ◽

Vol 21 (3) ◽

pp. 391-399 ◽

Cited By ~ 1

Author(s):

Elwood Buchman ◽

Harold J. Kullman ◽

George F. Margonis

Keyword(s):

Complement Fixation Test ◽

Complement Fixation ◽

Fixation Test

Download Full-text

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

Acta Endocrinologica ◽

10.1530/acta.0.062s113 ◽

1969 ◽

Vol 62 (1_Suppl) ◽

pp. S113-S133 ◽

Cited By ~ 2

Author(s):

Sam Brody

Keyword(s):

Complement Fixation Test ◽

Complement Fixation ◽

Research Work ◽

Fixation Test ◽

Years Of Experience ◽

Standard Practice ◽

Clinical Observations ◽

Technical Procedure ◽

Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Download Full-text

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2014-0052 ◽

2014 ◽

Vol 17 (2) ◽

pp. 367-369 ◽

Cited By ~ 3

Author(s):

K. Rypula ◽

A. Kumala ◽

P. Lis ◽

K. Niemczuk ◽

K. Płoneczka-Janeczko ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Complement Fixation Test ◽

Complement Fixation ◽

Fixation Test ◽

Reproductive Disorders ◽

Serum Samples ◽

Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

Download Full-text