Macromolecular organization of disk and plasma membranes of rod outer segment in the rat retina

1990 ◽  
Vol 15 ◽  
pp. S142
Author(s):  
Katsuyuki Miyaguchi ◽  
Takahiro Gotow ◽  
Paulo Hitonari Hashimoto
Keyword(s):  
Outer Segment ◽  
Plasma Membranes ◽  
Rat Retina ◽  
Rod Outer Segment ◽  
Macromolecular Organization

scholarly journals Differential distribution of proteins and lipids in detergent-resistant and detergent-soluble domains in rod outer segment plasma membranes and disks

2007 ◽  
Vol 0 (0) ◽  
pp. 071018045431009-??? ◽  
Author(s):  
Michael H. Elliott ◽  
Zack A. Nash ◽  
Nobuaki Takemori ◽  
Steven J. Fliesler ◽  
Mark E. McClellan ◽  
...  
Keyword(s):  
Outer Segment ◽  
Plasma Membranes ◽  
Differential Distribution ◽  
Rod Outer Segment

scholarly journals p19 detected in the rat retina and pineal gland is a guanylyl cyclase-activating protein (GCAP).

2002 ◽  
Vol 49 (4) ◽  
pp. 899-905
Author(s):  
Agnieszka Dejda ◽  
Izabela Matczak ◽  
Wojciech A Gorczyca
Keyword(s):  
Pineal Gland ◽  
Electrophoretic Mobility ◽  
Guanylyl Cyclase ◽  
Outer Segment ◽  
Mobility Shift ◽  
Rat Retina ◽  
Rod Outer Segment ◽  
First Time

The Ca(2+)-dependent activation of retina-specific guanylyl cyclase (retGC) is mediated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 microM) concentrations of Ca(2+). At low Ca(2+), immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat retinal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca(2+)-dependent electrophoretic mobility shift.

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