DNA Translocation of ATP-Dependent Chromatin Remodeling Factors Revealed by High-Resolution Optical Tweezers

2012 ◽  
pp. 3-28 ◽  
Author(s):  
Yongli Zhang ◽  
George Sirinakis ◽  
Greg Gundersen ◽  
Zhiqun Xi ◽  
Ying Gao
Keyword(s):  
High Resolution ◽  
Optical Tweezers ◽  
Chromatin Remodeling ◽  
Dna Translocation

scholarly journals DNA Translocation and Loop Formation Mechanism of Chromatin Remodeling by SWI/SNF and RSC

2006 ◽  
Vol 24 (4) ◽  
pp. 559-568 ◽  
Author(s):  
Yongli Zhang ◽  
Corey L. Smith ◽  
Anjanabha Saha ◽  
Stephan W. Grill ◽  
Shirley Mihardja ◽  
...  
Keyword(s):  
Chromatin Remodeling ◽  
Formation Mechanism ◽  
Loop Formation ◽  
Dna Translocation

scholarly journals Evidence for DNA Translocation by the ISWI Chromatin-Remodeling Enzyme

2003 ◽  
Vol 23 (6) ◽  
pp. 1935-1945 ◽  
Author(s):  
Iestyn Whitehouse ◽  
Chris Stockdale ◽  
Andrew Flaus ◽  
Mark D. Szczelkun ◽  
Tom Owen-Hughes
Keyword(s):  
Nucleic Acids ◽  
Atpase Activity ◽  
Chromatin Remodeling ◽  
Dna Translocation ◽  
Catalytic Core ◽  
Dna Translocase

ABSTRACT The ISWI proteins form the catalytic core of a subset of ATP-dependent chromatin-remodeling activities. Here, we studied the interaction of the ISWI protein with nucleosomal substrates. We found that the ability of nucleic acids to bind and stimulate the ATPase activity of ISWI depends on length. We also found that ISWI is able to displace triplex-forming oligonucleotides efficiently when they are introduced at sites close to a nucleosome but successively less efficiently 30 to 60 bp from its edge. The ability of ISWI to direct triplex displacement was specifically impeded by the introduction of 5- or 10-bp gaps in the 3′-5′ strand between the triplex and the nucleosome. In combination, these observations suggest that ISWI is a 3′-5′-strand-specific, ATP-dependent DNA translocase that may be capable of forcing DNA over the surface of nucleosomes.

scholarly journals Measuring DNA Translocation Forces through MoS 2 -Nanopores with Optical Tweezers

2017 ◽  
Vol 4 ◽  
pp. S168-S173 ◽  
Author(s):  
Sebastian Knust ◽  
Dennis Kreft ◽  
Roland Hillmann ◽  
Andreas Meyer ◽  
Martina Viefhues ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Dna Translocation

Chromatin remodeling through directional DNA translocation from an internal nucleosomal site

2005 ◽  
Vol 12 (9) ◽  
pp. 747-755 ◽  
Author(s):  
Anjanabha Saha ◽  
Jacqueline Wittmeyer ◽  
Bradley R Cairns
Keyword(s):  
Chromatin Remodeling ◽  
Dna Translocation
Sign in / Sign up

Export Citation Format

Share Document