SYNTHESIS, TRANSPORT, AND LIFE-SPAN OF AMINE STORAGE GRANULES IN SYMPATHETIC ADRENERGIC NEURONS

1969 ◽  
pp. 153-174 ◽  
Author(s):  
ANNICA DAHLSTRÖM
Keyword(s):  
Life Span ◽  
Adrenergic Neurons ◽  
Amine Storage ◽  
Storage Granules ◽  
Amine Storage Granules

scholarly journals Transport and storage of serotonin by thrombin-treated platelets.

1975 ◽  
Vol 65 (2) ◽  
pp. 359-372 ◽  
Author(s):  
H J Reimers ◽  
D J Allen ◽  
I A Feuerstein ◽  
J F Mustard
Keyword(s):  
Storage Capacity ◽  
Platelet Membrane ◽  
Transport Rate ◽  
Primary Reason ◽  
High Concentration ◽  
Serotonin Content ◽  
Amine Storage ◽  
Storage Granules ◽  
And Storage ◽  
Amine Storage Granules

Repeated thrombin treatment of washed platelets prepared from rabbits can decrease the serotonin content of the platelets by about 80%. When these platelets are deaggregated they reaccumulate serotonin but their storage capacity for serotonin is reduced by about 60%. If thrombin-pretreated platelets are allowed to equilibrate with a high concentration of serotonin (123 mu M), they release a smaller percentage of their total serotonin upon further thrombin treatment, in comparison with the percentage of serotonin released from control platelets equilibrated with the same concentration of serotonin calculations indicate that in thrombin-treated platelets reequilibrated with serotonin, two-thirds of the serotonin is in the granule compartment and one-third is in the extragranular compartment, presumably the cytoplasm. Analysis of the exchange of serotonin between the suspending fluid and the platelets showed that thrombin treatment does not alter the transport rate of serotonin across the platelet membrane and does not cause increased diffusion of serotonin from the platelets into the suspending fluid. The primary reason for the reduced serotonin accumulation by the thrombin-treated platelets appears to be loss of amine storage granules or of the storage capacity within the granules.

Depletion of Platelet Amine Storage Granules by the Antithrombotic Agent, Suloctidil

1977 ◽  
Vol 38 (04) ◽  
pp. 1010-1017 ◽  
Author(s):  
David C. B. Mills ◽  
Donald E. Macfarlane
Keyword(s):  
Energy Balance ◽  
Animal Models ◽  
Human Platelet ◽  
Platelet Rich Plasma ◽  
Oxidation Products ◽  
Antithrombotic Agent ◽  
Amine Storage ◽  
Storage Granules ◽  
Amine Storage Granules

SummarySuloctidil, a compound with antithrombotic properties in animal models, causes depletion of human platelet serotonin stores during 1–5 hr incubation with platelet-rich plasma in vitro. This effect is not attended by leakage of cytoplasmic nucleotides or by alterations in cyclic AMP metabolism, malondialdehyde production or energy balance. 5HT uptake was also inhibited but uptake of adenine was not. 5HT released by suloctidil appeared in the supernatant as oxidation products, though the amine also accumulated when re-uptake was blocked by imipramine.

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