The article presents the previous and current methods and markers used for estimation of human age. The analysis of biological material recovered from the scene of the event makes it possible to estimate the age of a person who deposited traces. The new methods allow determining the depositor’s age, based on biological traces commonly found at the scene, such as blood, saliva or sperm, with an accuracy of a few years. The previously used age estimation techniques required larger quantities of biological material, whereas their prediction error amounted to even several decades.