Microfluidic systems for drug discovery, pharmaceutical analysis, and diagnostic applications

2021 ◽  
pp. 261-327
Author(s):  
Dawei Ding ◽  
Sol Park ◽  
Jaspreet Singh Kochhar ◽  
Sui Yung Chan ◽  
Pei Shi Ong ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Pharmaceutical Analysis ◽  
Microfluidic Systems ◽  
Diagnostic Applications

scholarly journals Using Aptamers as Capture Reagents in Bead-Based Assay Systems for Diagnostics and Hit Identification

2006 ◽  
Vol 11 (7) ◽  
pp. 773-781 ◽  
Author(s):  
Peter Porschewski ◽  
Mira A.-M. Grättinger ◽  
Kerstin Klenzke ◽  
Anja Erpenbach ◽  
Michael R. Blind ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Protein Complex ◽  
Test System ◽  
Serum Samples ◽  
Protein Target ◽  
Specificity And Sensitivity ◽  
Diagnostic Applications ◽  
Hit Identification ◽  
Synthetic Oligonucleotides ◽  
Xmap Technology

Most applications of xMAP™ (Luminex®) bead-based assay technology in diagnostics and drug discovery use immobilized antigens or antibodies. Here the authors describe the development of novel assay systems in which synthetic oligonucleotides that specifically bind and inhibit other biomolecules—so-called aptamers—are directly immobilized on beads. The robustness, specificity, and sensitivity of aptamer-based assays were demonstrated in a test system that detected human α-thrombin in serum samples. xMAP technology was also adapted to competitive screening formats where an aptamer/protein complex was disrupted by a functionally analogous competitor. The results indicate that such assays are excellently suited for diagnostic applications or drug screening, where aptamers serve as competitive binding probes for the identification of small-molecule hits. These methods should be transferable to a large number of applications because specific aptamers can be rapidly generated for almost any protein target.

scholarly journals Pharmaceutical analysis combined with in-silico therapeutic and toxicological profiling on zileuton and its impurities to assist in modern drug discovery

2020 ◽  
Vol 179 ◽  
pp. 112982
Author(s):  
Saurabh B. Ganorkar ◽  
Yvan Vander Heyden ◽  
Atul A. Shirkhedkar ◽  
Deepak K. Lokwani ◽  
Dinesh M. Dhumal ◽  
...  
Keyword(s):  
Drug Discovery ◽  
In Silico ◽  
Pharmaceutical Analysis ◽  
Modern Drug

Current trends in modern pharmaceutical analysis for drug discovery

2003 ◽  
Vol 8 (19) ◽  
pp. 889-897 ◽  
Author(s):  
Hwee-Ling Koh ◽  
Wai-Ping Yau ◽  
Pei-Shi Ong ◽  
Akhil Hegde
Keyword(s):  
Drug Discovery ◽  
Pharmaceutical Analysis ◽  
Current Trends

Aspiration cytology and transmission electron microscopy (TEM): A novel tissue-collection technique with useful diagnostic applications

1984 ◽  
Vol 42 ◽  
pp. 90-93
Author(s):  
J.A. Maksem ◽  
C. VanDyke ◽  
H.W. Carter ◽  
C.F. Galang
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Diagnostic Technique ◽  
Needle Aspiration ◽  
Large Series ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Transmission Electron ◽  
Diagnostic Applications ◽  
Microscopic Slide

In the last decade fine needle aspiraration biopsy has gained recognition as a valuable diagnostic technique, and its benefits have been demonstrated in large series of patients with almost every type of tumor (1,2). The usual way to collect cellular material from needle-aspiration biopsies is to discharge the needle and syringe contents onto a microscopic slide and smear the material with another slide. The entire specimen is contained on the slides prepared at the time of biopsy. Serious technical difficulties are inherent to this method. 1) Inconsistent fixation, 2) drying artifact, 3) loss of tissue fragments, 4) inability to confirm impressions by a “second method”, and 5) retention of significant diagnostic material in the needle hub. Our technique avoids these difficulties.

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