Acoustic-Field Beamforming-Based Generalized Coherence Factor for Handheld Ultrasound

Handheld ultrasound devices have been widely used for diagnostic applications. The use of the acoustic-field beamforming (AFB) method has been proposed for handheld ultrasound to reduce electricity consumption and avoid battery and unwanted heat issues. However, the image quality, such as the contrast ratio and contrast-to-noise-ratio, are poorer with this technique than with the conventional delay-and-sum method. To address the problems associated with the worse image quality in AFB imaging, in this paper we propose the use of an AFB-based generalized coherence factor (GCF) technique, in which the GCF weighting developed for adaptive beamforming is extended to AFB. Simulation data, experimental results, and in vivo testing verified the efficacy of our proposed AFB-based GCF technique.

Aptamer-Enabled Nanomaterials for Therapeutics, Drug Targeting and Imaging

A wide variety of nanomaterials have emerged in recent years with advantageous properties for a plethora of therapeutic and diagnostic applications. Such applications include drug delivery, imaging, anti-cancer therapy and radiotherapy. There is a critical need for further components which can facilitate therapeutic targeting, augment their physicochemical properties, or broaden their theranostic applications. Aptamers are single-stranded nucleic acids which have been selected or evolved to bind specifically to molecules, surfaces, or cells. Aptamers can also act as direct biologic therapeutics, or in imaging and diagnostics. There is a rich field of discovery at the interdisciplinary interface between nanomaterials and aptamer science that has significant potential across biomedicine. Herein, we review recent progress in aptamer-enabled materials and discuss pending challenges for their future biomedical application.

In-Utero Neurotoxicity of Nanoparticles

The unique physicochemical properties of nanoparticles (NPs) make them widely used in cosmetics, medicines, food additives, and antibacterial and antiviral compounds. NPs are also used in therapy and diagnostic applications. Depending on their origin, the NPs are commonly classified as naturally occurring and synthetic or anthropogenic NPs. Naturally occurring nanoparticles can be formed by many physical, chemical, and biological processes occurring in all spheres of the earth. However, synthetic NPs are specifically designed or unintentionally produced by different human activities. Owing to their nano size and special properties, the engineered NPs can enter the human body through different routes such as dermal penetration, intravenous injection and inhalation. NPs may accumulate in various tissues and organs including the brain. Indiscriminate use of NP is a matter concern due to the dangers of NP exposure to living organisms. It is possible for NPs to cross the placental barrier, and adversely affect the developing fetus, posing a health hazard in them by causing neurodevelopmental toxicity. Thus, NP-induced neurotoxicity is a topic that demands attention at the maternal-fetal interface. This chapter summarizes the routes by which NPs circumvent the blood-brain barrier, including recent investigations about NPs’ neurotoxicity as well as possible mechanisms involved in neural fetotoxicity.

CRISPR-Cas System: A Promising Diagnostic Tool for Covid-19

More than a year has passed since the beginning of the 2019 novel coronavirus diseases (COVID-19) pandemic which has created massive problems globally affecting all aspects of people's life. Due to the emergence of new strains of the SARS-CoV-2, pandemic risk still remains, despite the start of vaccination. Therefore, rapid diagnostic tests are essential to control infection, improve clinical care and stop the spread of the disease. Recently CRISPR-based diagnostic tools have facilitated rapid diagnostic. Here, we review the diagnostic applications of CRISPR-Cas system in COVID-19.

DNA Origami-Templated Bimetallic Nanostar Assemblies for Ultra-Sensitive Detection of Dopamine

The abundance of hotspots tuned via precise arrangement of coupled plasmonic nanostructures highly boost the surface-enhanced Raman scattering (SERS) signal enhancements, expanding their potential applicability to a diverse range of applications. Herein, nanoscale assembly of Ag coated Au nanostars in dimer and trimer configurations with tunable nanogap was achieved using programmable DNA origami technique. The resulting assemblies were then utilized for SERS-based ultra-sensitive detection of an important neurotransmitter, dopamine. The trimer assemblies were able to detect dopamine with picomolar sensitivity, and the assembled dimer structures achieved SERS sensitivity as low as 1 fM with a limit of detection of 0.225 fM. Overall, such coupled nanoarchitectures with superior plasmon tunability are promising to explore new avenues in biomedical diagnostic applications.

Flow Control in Passive 3D Paper-Based Microfluidic Pump by Variable Porosity

Active pumps are often used in microfluidic devices for programmable fluid flowrate in a microchannel. Active pumps have some drawbacks due to their large size and requirement of external power. To overcome them, a new class of passive pumps based on capillary action in cellulose material, known as paper-based microfluidic pumps, has recently been explored. In this study, fluid flow in 3D paper-based pumps was investigated using flowrate measurements in microchannels. In order to develop 3D cylindrical pumps, Whatman filter paper grade 1 was shredded, mixed with water, molded and dried. The patterned serpentine channel was created using a CO2 Laser Cutting/Engraving machine. The 3D paper-based pump was integrated with microfluidic channel. The effect of paper pumps of different porosities on the fluid flowrate through a serpentine microchannel was investigated. It was found that flowrate of the fluid flowing through the channel increases with an increase in the pump’s porosity. Moreover, these pumps have the ability to transport larger volumes of liquid with improved flowrate, programmability and control, in addition to being inexpensive and simple to design and fabricate. These 3D pumps will help researchers move closer to developing an effective miniaturized diagnostic platform for point-of-care (POC) diagnostic applications.

Case Series: Superficial Sonography in Urgent Care

Sonography is an essential imaging modality, known for its real-time capabilities, relative low cost, and multiple diagnostic applications. Although there are protocols which are well-established for specific examinations, there are not clear guidelines for sonographic examinations of soft tissues. Many sonographers have to establish their own imaging protocols when completing a soft tissue examination. This case series details five soft tissue examinations that represent just a few of the clinical requests generated in an Urgent Care (UC) facility. Since UC usage has increased over the past several years, it appears likely that any sonographer working in such outpatient settings will benefit from as much exposure to soft tissue examinations as possible.

2D Materials-Based Aptamer Biosensors: Present Status and Way Forward

: Current advances in constructing functional nanomaterials and elegantly designed nanostructures have opened up new possibilities for the fabrication of viable field biosensors. Two-dimensional materials (2DMs) have fascinated much attention due to their chemical, optical, physicochemical, and electronic properties. They are ultrathin nanomaterials with unique properties such as high surface-to-volume ratio, surface charge, shape, high anisotropy, and adjustable chemical functionality. 2DMs such as graphene-based 2D materials, Silicate clays, layered double hydroxides (LDHs), MXenes, transition metal dichalcogenides (TMDs), and transition metal oxides (TMOs) offer intensified physicochemical and biological functionality and have proven to be very promising candidates for biological applications and technologies. 2DMs have a multivalent structure that can easily bind to single-stranded DNA/RNA (aptamers) through covalent, non-covalent, hydrogen bond, and π-stacking interactions, whereas aptamers have a small size, excellent chemical stability, and low immunogenicity with high affinity and specificity. This review discussed the potential of various 2D material-based aptasensor for diagnostic applications, e.g., protein detection, environmental monitoring, pathogens detection, etc.