Water containing polyvinyl nitrate was experimentally assessed on the basis of organoleptic, general sanitary and toxicological indicators of harmfulness. It was established that that the compound did not change water organoleptic properties but produced a negative impact on viability of saprophytic microflora , nitrification processes and biochemical oxygen demand. Besides, in tests on animals. the substance caused acute, sub-acute and chronic toxicity. Based on those signs of harmfulness, threshold levels of exposure were established. Data obtained were taken into account for substantiation of MAC (Maximum allowable concentration) of polyvinyl nitrate in water bodies.