Isotope Labeling of Proteins in Insect Cells

2015 ◽  
pp. 245-288 ◽  
Author(s):  
Lukasz Skora ◽  
Binesh Shrestha ◽  
Alvar D. Gossert
Keyword(s):  
Insect Cells ◽  
Isotope Labeling

Affordable uniform isotope labeling with 2H, 13C and 15N in insect cells

2015 ◽  
Vol 62 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Agnieszka Sitarska ◽  
Lukasz Skora ◽  
Julia Klopp ◽  
Susan Roest ◽  
César Fernández ◽  
...  
Keyword(s):  
Insect Cells ◽  
Isotope Labeling ◽  
Uniform Isotope Labeling

Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells

2005 ◽  
Vol 31 (4) ◽  
pp. 343-349 ◽  
Author(s):  
André Strauss ◽  
Francis Bitsch ◽  
Gabriele Fendrich ◽  
Patrick Graff ◽  
René Knecht ◽  
...  
Keyword(s):  
Insect Cells ◽  
Isotope Labeling ◽  
Abl Kinase ◽  
Infected Insect ◽  
Uniform Isotope Labeling

scholarly journals Isotope Labeling in Insect Cells

2011 ◽  
pp. 37-54 ◽  
Author(s):  
Krishna Saxena ◽  
Arpana Dutta ◽  
Judith Klein-Seetharaman ◽  
Harald Schwalbe
Keyword(s):  
Insect Cells ◽  
Isotope Labeling

A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility

2011 ◽  
Vol 51 (4) ◽  
pp. 449-456 ◽  
Author(s):  
Alvar D. Gossert ◽  
Alexandra Hinniger ◽  
Sascha Gutmann ◽  
Wolfgang Jahnke ◽  
André Strauss ◽  
...  
Keyword(s):  
Amino Acid ◽  
Insect Cells ◽  
Isotope Labeling ◽  
Amino Acid Type ◽  
High Reproducibility ◽  
Acid Type ◽  
Simple Protocol ◽  
Selective Isotope Labeling

An improved protocol for amino acid type-selective isotope labeling in insect cells

2017 ◽  
Vol 68 (4) ◽  
pp. 237-247 ◽  
Author(s):  
Yaqun Zhang ◽  
Hui Wei ◽  
Dianlin Xie ◽  
Deepa Calambur ◽  
Andrew Douglas ◽  
...  
Keyword(s):  
Amino Acid ◽  
Insect Cells ◽  
Isotope Labeling ◽  
Amino Acid Type ◽  
Acid Type ◽  
Selective Isotope Labeling

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

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