scholarly journals In vivo confocal microscopic images of atypical amiodarone-induced keratopathy in patient with epithelial basement membrane dystrophy

2021 ◽  
pp. 101105
Author(s):  
Hidenori Inoue ◽  
Koji Toriyama ◽  
Takeshi Joko ◽  
Atsushi Shiraishi
Keyword(s):  
Basement Membrane ◽  
Microscopic Images ◽  
Epithelial Basement Membrane ◽  
Epithelial Basement

scholarly journals A Surgical Cryoprobe for Targeted Transcorneal Freezing and Endothelial Cell Removal

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Alina Akhbanbetova ◽  
Shinichiro Nakano ◽  
Stacy L. Littlechild ◽  
Robert D. Young ◽  
Madara Zvirgzdina ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Basement Membrane ◽  
Endothelial Damage ◽  
Epithelial Basement Membrane ◽  
Fibril Structure ◽  
Consistent Manner ◽  
Stromal Edema ◽  
Epithelial Basement

Purpose. To examine the effects of transcorneal freezing using a new cryoprobe designed for corneal endothelial surgery. Methods. A freezing console employing nitrous oxide as a cryogen was used to cool a series of different cryoprobe tip designs made of silver for high thermal conductivity. In vitro studies were conducted on 426 porcine corneas, followed by preliminary in vivo investigations on three rabbit corneas. Results. The corneal epithelium was destroyed by transcorneal freezing, as expected; however, the epithelial basement membrane remained intact. Reproducible endothelial damage was optimally achieved using a 3.4 mm diameter cryoprobe with a concave tip profile. Stromal edema was seen in the pre-Descemet’s area 24 hrs postfreeze injury, but this had been resolved by 10 days postfreeze. A normal collagen fibril structure was seen 1 month postfreeze, concurrent with endothelial cell repopulation. Conclusions. Transcorneal freezing induces transient posterior stromal edema and some residual deep stromal haze but leaves the epithelial basement membrane intact, which is likely to be important for corneal re-epithelialization. Localized destruction of the endothelial monolayer was achieved in a consistent manner with a 3.4 mm diameter/concave profile cryoprobe and represents a potentially useful approach to remove dysfunctional corneal endothelial cells from corneas with endothelial dysfunction.

Kalinin: A new epithelial basement membrane adhesion molecule

1991 ◽  
Vol 49 ◽  
pp. 178-179
Author(s):  
Douglas R. Keene ◽  
Gregory P. Lunstrum ◽  
Patricia Rousselle ◽  
Robert E. Burgeson
Keyword(s):  
Basement Membrane ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Keratinocytes ◽  
Positive Staining ◽  
Human Amnion ◽  
Epithelial Basement Membrane ◽  
Type Vii Collagen ◽  
Keratinocyte Cell ◽  
Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

Increased glycosylation of human lens epithelial basement membrane in diabetes mellitus

1994 ◽  
Vol 8 (1) ◽  
pp. 26 ◽  
Author(s):  
Dong H. Shin ◽  
Shlomo S. Mandel ◽  
Jin H. Lee ◽  
Brett Ernst ◽  
Bruce L. Newman ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Basement Membrane ◽  
Human Lens ◽  
Epithelial Basement Membrane ◽  
Epithelial Basement
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