scholarly journals The cytochrome ba3 oxidase from Thermus thermophilus does not generate a tryptophan radical during turnover: Implications for the mechanism of proton pumping

2015 ◽  
Vol 1847 (10) ◽  
pp. 1093-1100 ◽  
Author(s):  
Angela Paulus ◽  
Carolin Werner ◽  
Bernd Ludwig ◽  
Simon de Vries
Keyword(s):  
Thermus Thermophilus ◽  
Proton Pumping ◽  
Cytochrome Ba3

scholarly journals Mutation of a single residue in the ba3 oxidase specifically impairs protonation of the pump site

2015 ◽  
Vol 112 (11) ◽  
pp. 3397-3402 ◽  
Author(s):  
Christoph von Ballmoos ◽  
Nathalie Gonska ◽  
Peter Lachmann ◽  
Robert B. Gennis ◽  
Pia Ädelroth ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Time Constant ◽  
Thermus Thermophilus ◽  
Proton Translocation ◽  
Proton Pumping ◽  
Wild Type ◽  
Structural Variant ◽  
In The Wild ◽  
Membrane Bound ◽  
Proton Uptake

The ba3-type cytochrome c oxidase from Thermus thermophilus is a membrane-bound protein complex that couples electron transfer to O2 to proton translocation across the membrane. To elucidate the mechanism of the redox-driven proton pumping, we investigated the kinetics of electron and proton transfer in a structural variant of the ba3 oxidase where a putative “pump site” was modified by replacement of Asp372 by Ile. In this structural variant, proton pumping was uncoupled from internal electron transfer and O2 reduction. The results from our studies show that proton uptake to the pump site (time constant ∼65 μs in the wild-type cytochrome c oxidase) was impaired in the Asp372Ile variant. Furthermore, a reaction step that in the wild-type cytochrome c oxidase is linked to simultaneous proton uptake and release with a time constant of ∼1.2 ms was slowed to ∼8.4 ms, and in Asp372Ile was only associated with proton uptake to the catalytic site. These data identify reaction steps that are associated with protonation and deprotonation of the pump site, and point to the area around Asp372 as the location of this site in the ba3 cytochrome c oxidase.

scholarly journals Exploring the proton pump and exit pathway for pumped protons in cytochrome ba3 from Thermus thermophilus

2012 ◽  
Vol 109 (14) ◽  
pp. 5259-5264 ◽  
Author(s):  
H.-Y. Chang ◽  
S. K. Choi ◽  
A. S. Vakkasoglu ◽  
Y. Chen ◽  
J. Hemp ◽  
...  
Keyword(s):  
Proton Pump ◽  
Thermus Thermophilus ◽  
Cytochrome Ba3

scholarly journals Electron paramagnetic resonance studies of the soluble CuA protein from the cytochrome ba3 of Thermus thermophilus

1996 ◽  
Vol 71 (5) ◽  
pp. 2823-2829 ◽  
Author(s):  
M. Karpefors ◽  
C.E. Slutter ◽  
J.A. Fee ◽  
R. Aasa ◽  
B. Källebring ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Thermus Thermophilus ◽  
Paramagnetic Resonance ◽  
Cytochrome Ba3 ◽  
Electron Paramagnetic

Spectroscopic characterization of the Fe(II)a·CN complex of Thermus thermophilus cytochrome ba3: A novel reaction of a terminal oxidase with cyanide

1991 ◽  
Vol 43 (2-3) ◽  
pp. 352
Author(s):  
Kristene K. Surerus ◽  
W.Anthony Oertling ◽  
Chaoliang Fan ◽  
Ólöf Einarsdóttir ◽  
R.Brian Dyer ◽  
...  
Keyword(s):  
Thermus Thermophilus ◽  
Spectroscopic Characterization ◽  
Terminal Oxidase ◽  
Cytochrome Ba3

scholarly journals Electrical current generation and proton pumping catalyzed by the ba 3 -type cytochrome c oxidase from Thermus thermophilus

FEBS Letters ◽  
1998 ◽  
Vol 434 (1-2) ◽  
pp. 17-22 ◽  
Author(s):  
Aimo Kannt ◽  
Tewfik Soulimane ◽  
Gerhard Buse ◽  
Anja Becker ◽  
Ernst Bamberg ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Thermus Thermophilus ◽  
Electrical Current ◽  
Proton Pumping ◽  
Current Generation
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