Structural characterization of the Borrelia burgdorferi outer surface protein BBA73 implicates dimerization as a functional mechanism

2013 ◽  
Vol 434 (4) ◽  
pp. 848-853 ◽  
Author(s):  
Kalvis Brangulis ◽  
Ivars Petrovskis ◽  
Andris Kazaks ◽  
Viesturs Baumanis ◽  
Kaspars Tars
Keyword(s):  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Outer Surface ◽  
Surface Protein ◽  
Functional Mechanism ◽  
Outer Surface Protein

Multiple cross-reactive seif-ligands for Borrelia burgdorferi outer surface protein A (OspA)-specific HLA-DR4-restricted T Cells

1999 ◽  
Vol 289 (5-7) ◽  
pp. 673 ◽  
Author(s):  
Bert Maier ◽  
Marc Molinger ◽  
Andrew P. Cope ◽  
Lars Fugger ◽  
Jens Schneider-Mergener ◽  
...  
Keyword(s):  
T Cells ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Protein A ◽  
Surface Protein ◽  
Outer Surface Protein A ◽  
Outer Surface Protein ◽  
Multiple Cross

scholarly journals Constitutive Expression of Outer Surface Protein C Diminishes the Ability of Borrelia burgdorferi To Evade Specific Humoral Immunity

2006 ◽  
Vol 74 (9) ◽  
pp. 5177-5184 ◽  
Author(s):  
Qilong Xu ◽  
Sunita V. Seemanapalli ◽  
Kristy McShan ◽  
Fang Ting Liang
Keyword(s):  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Protein C ◽  
Severe Combined Immunodeficiency ◽  
Constitutive Expression ◽  
Surface Protein ◽  
Start Codon ◽  
Chronic Infections ◽  
Translational Initiation ◽  
Outer Surface Protein

ABSTRACT The Lyme disease spirochete Borrelia burgdorferi reduces the expression of outer surface protein C (OspC) in response to the development of an anti-OspC humoral response, leading to the hypothesis that the ability to repress OspC expression is critical for the pathogen to proceed to chronic infection. B. burgdorferi was genetically modified to constitutively express OspC by introducing an extra ospC copy fused with the borrelial flagellar gene (flaB) promoter. Such a genetic modification did not reduce infectivity or pathogenicity in severe combined immunodeficiency mice but resulted in clearance of infection by passively transferred OspC antibody. Spirochetes with constitutive ospC expression were unable to establish chronic infections in immunocompetent mice unless they had undergone very destructive mutations in the introduced ospC copy. Two escape mutants were identified; one had all 7 bp deleted between the putative ribosome-binding site and the start codon, ATG, causing a failure in translational initiation, and the other mutant had an insertion of 2 bp between nucleotides 315 and 316, resulting in a nonsense mutation at codon 108. Thus, the ability of B. burgdorferi to repress ospC expression during mammalian infection allows the pathogen to avoid clearance and to preserve the integrity of the important gene for subsequent utilization during its enzootic life cycle.

scholarly journals Outer Surface Protein C Is a Dissemination-Facilitating Factor of Borrelia burgdorferi during Mammalian Infection

PLoS ONE ◽  
2010 ◽  
Vol 5 (12) ◽  
pp. e15830 ◽  
Author(s):  
Sunita V. Seemanapalli ◽  
Qilong Xu ◽  
Kristy McShan ◽  
Fang Ting Liang
Keyword(s):  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Protein C ◽  
Surface Protein ◽  
Outer Surface Protein ◽  
Mammalian Infection

Immunogenicity of a recombinant Borrelia burgdorferi outer surface protein A vaccine against Lyme disease in children

1999 ◽  
Vol 135 (5) ◽  
pp. 575-579 ◽  
Author(s):  
Henry M. Feder ◽  
Jeri Beran ◽  
Christian Van Hoecke ◽  
Betsy Abraham ◽  
Norbert De Clercq ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Protein A ◽  
Surface Protein ◽  
Outer Surface Protein A ◽  
Outer Surface Protein
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