Electrophoresis of secretions collected from Globodera
pallida revealed a smeared region between 25 and 50 kDa, and a
single band of <20 kDa. The secretions were used to raise an antiserum
(LW1). Immunoblotting of parasite homogenates
with LW1 differentiated G. pallida from its sibling species
G. rostochiensis and revealed differences between different
populations of G. pallida and G. rostochiensis.
Indirect immunofluorescence studies with LW1 indicated that at least some
of the secretions were surface localized and that antibody binding to the
nematode surface was periodate sensitive.
Periodate sensitivity indicated that these differences could be due to
glycosylation differences. Glycosylation differences
were also detected by blotting nematode homogenates with the lectin wheat
germ agglutinin (WGA). WGA was also able
to differentiate between G. rostochiensis which gave 2 bands at
130 kDa and 110 kDa, and G. pallida which produced 2
bands present at 120 kDa and 110 kDa. Further localization studies
using immunoelectron microscopy demonstrated that
antibody binding could be seen to secretions found in the pump chamber
of
the metacorpal bulb at the base of the stylet.
From further specimens it could be observed that the contents of the
subventral glands were heavily labelled, indicating
that the material seen in the metacorpal bulb had originated from the
subventral glands.
Characterization of Plant-Parasitic Nematode Communities Associated With Brinjal Crops in the Nagole Area of Rangareddy District, Hyderabad, Telangana, India (2011-2012)
