Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens

2008 ◽  
Vol 99 (11) ◽  
pp. 4597-4602 ◽  
Author(s):  
Dhanya Gangadharan ◽  
Swetha Sivaramakrishnan ◽  
K. Madhavan Nampoothiri ◽  
Rajeev K. Sukumaran ◽  
Ashok Pandey
2011 ◽  
Vol 236-238 ◽  
pp. 2323-2326 ◽  
Author(s):  
Jun Liang Sun ◽  
Xin Hong Liang ◽  
Jie Zeng ◽  
Guang Lei Li ◽  
Jun Liang Sun

The aim of this work was to optimize the process parameters through the statistical approach for the production of alpha amylase by Bacillus subtilis ZJF-1A5 in submerged fermentation (SMF). The process parameters influencing the enzyme production were identified by using response surface methodology. Among the variables screened, the temperature and time were most significant and showed a positive interaction. The optimum levels were: temperature 35.8 °C, pH 5.03 and time 54h and under these conditions α-amylase yield was up to 191.15 U/mL.


2015 ◽  
Vol 9 (3) ◽  
pp. 120-128 ◽  
Author(s):  
AHMAD WIBISANA ◽  
◽  
WAHONO SUMARYONO ◽  
TJAHJANI MIRAWATI SUDIRO ◽  
PRATIWI PUDJILESTARI SUDARMONO ◽  
...  

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