Response surface methodology as an approach for optimization of α-amylase production by the new isolated thermotolerant Bacillus licheniformis WF67 strain in submerged fermentation

2021 ◽  
Vol 32 ◽  
pp. 101944
Author(s):  
Walid F. Saad ◽  
Abdelmageed M. Othman ◽  
Medhat Abdel-Fattah ◽  
Maged S. Ahmad
2011 ◽  
Vol 236-238 ◽  
pp. 2323-2326 ◽  
Author(s):  
Jun Liang Sun ◽  
Xin Hong Liang ◽  
Jie Zeng ◽  
Guang Lei Li ◽  
Jun Liang Sun

The aim of this work was to optimize the process parameters through the statistical approach for the production of alpha amylase by Bacillus subtilis ZJF-1A5 in submerged fermentation (SMF). The process parameters influencing the enzyme production were identified by using response surface methodology. Among the variables screened, the temperature and time were most significant and showed a positive interaction. The optimum levels were: temperature 35.8 °C, pH 5.03 and time 54h and under these conditions α-amylase yield was up to 191.15 U/mL.


2019 ◽  
Vol 16 (6) ◽  
pp. 190
Author(s):  
Võ Hồng Phượng ◽  
Nguyễn Hoàng Tuấn ◽  
Phạm Thị Huyền Diệu ◽  
Nguyễn Thị Lan Chi ◽  
Lê Thị Bích Thủy ◽  
...  

The production of biomass from Bacillus licheniformsis B1 was investigated in submerged fermentation. Optimization of culture medium was carried out by using response surface methodology (RSM). The B1 biomass production was also significantly affected by dextrose, soya, CaCl2. In addition, while the optimum cultivation parameters were (g/L): 44.026 of dextrose, 34.025 of soya and 3.491 of CaCl2, the prediction of biomass production of B1 was 6.05 x 109 CFU ml-1.


2018 ◽  
Vol 55 (7) ◽  
pp. 2579-2586 ◽  
Author(s):  
Yuwan Wang ◽  
Mingyue Zhang ◽  
Zhengzhu Zhang ◽  
Jun Jiang ◽  
Xueling Gao ◽  
...  

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