High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation

2013 ◽  
Vol 127 ◽  
pp. 291-297 ◽  
Author(s):  
T.Q. Lan ◽  
Roland Gleisner ◽  
J.Y. Zhu ◽  
Bruce S. Dien ◽  
Ronald E. Hector
Keyword(s):  
Ethanol Production ◽  
Lodgepole Pine ◽  
High Titer ◽  
Enzymatic Saccharification

scholarly journals A Hydrotrope Pretreatment for Stabilized Lignin Extraction and High Titer Ethanol Production

2022 ◽  
Author(s):  
Hairui Ji ◽  
Le Wang ◽  
Furong Tao ◽  
Zhipeng Yao ◽  
Xuezhi Li ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Ethanol Yield ◽  
High Titer ◽  
Enzymatic Saccharification ◽  
Value Added ◽  
Enzymatic Digestion ◽  
Lignin Extraction ◽  
C 30 ◽  
Theoretical Ethanol Yield ◽  
Light Color

Abstract The biomass pretreatment strategies using organic acids facilitate lignin removal and enhance the enzymatic digestion of cellulose. However, lignin always suffers a severe and irreversible condensation. The newly generated C-C bonds dramatically affect its further upgrading. In this study, we used a recyclable hydrotrope (p-Toluenessulfonic acid, p-TsOH) to dissolve lignin under mild condition and stabilized lignin with a quenching agent (formaldehyde, FA) during extraction, achieving both value-added lignin extraction and efficient enzymatic saccharification of cellulose. Approximately 63.7% of lignin was dissolved by 80% (wt. %) p-TsOH with 1.5% FA addition at 80 o C, 30 min. The obtained lignin was characterized by FTIR spectroscopy, TGA, 2D HSQC NMR spectroscopy, and GPC. The results indicated that the extracted lignin exhibited excellent properties, such as light color, a low molecular weight (Mw, 5371 g/mol), and a narrow polydispersity (Mw/Mn, 1.63). The pretreated substrate was converted to ethanol via a quasi-simultaneous saccharification and fermentation process (Q-SSF). After fermentation of 60 h, the ethanol concentration reached 38.7±3.3 g/L which was equivalent to a theoretical ethanol yield of 82.9±2.2% based on the glucan content, while the residual glucose concentration was only 4.69±1.4 g/L. In short, this pretreatment strategy protected lignin to form new C-C linkages and improved the enzymatic saccharification of glucan for high-titer ethanol production.

Comparisons of high titer ethanol production and lignosulfonate properties by SPORL pretreatment of lodgepole pine at two temperatures

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27030-27038 ◽  
Author(s):  
Haifeng Zhou ◽  
Shao-Yuan Leu ◽  
Xiaolei Wu ◽  
J. Y. Zhu ◽  
Roland Gleisner ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Mountain Pine Beetle ◽  
Lodgepole Pine ◽  
High Titer ◽  
Wood Chips ◽  
Pine Wood ◽  
Pine Beetle ◽  
Two Temperatures ◽  
Pine Wood Chips ◽  
Pretreatment Severity

Mountain pine beetle killed lodgepole pine wood chips were pretreated by SPORL at 180 °C for 25 min and 165 °C for 75 min using the same chemical loadings, which represent the same pretreatment severity.

scholarly journals Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production

2017 ◽  
Vol 225 ◽  
pp. 191-198 ◽  
Author(s):  
Jose A. Pérez-Pimienta ◽  
Alejandra Vargas-Tah ◽  
Karla M. López-Ortega ◽  
Yessenia N. Medina-López ◽  
Jorge A. Mendoza-Pérez ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ethanol Production ◽  
Enzymatic Saccharification

Sequential acid and enzymatic saccharification of steam exploded cotton stalk and subsequent ethanol production using Scheffersomyces stipitis NCIM 3498

2018 ◽  
Vol 125 ◽  
pp. 462-467 ◽  
Author(s):  
Praveen Kumar Keshav ◽  
Chandrasekhar Banoth ◽  
Archana Anthappagudem ◽  
Venkateswar Rao Linga ◽  
Bhima Bhukya
Keyword(s):  
Ethanol Production ◽  
Enzymatic Saccharification ◽  
Cotton Stalk ◽  
Scheffersomyces Stipitis

Fibrillating wood chips to facilitate high-valued lignin extraction and high titer ethanol production

2020 ◽  
Vol 146 ◽  
pp. 112153
Author(s):  
Yan Wu ◽  
Hairui Ji ◽  
Xingxiang Ji ◽  
Zhongjian Tian ◽  
Jiachuan Chen
Keyword(s):  
Ethanol Production ◽  
High Titer ◽  
Wood Chips ◽  
Lignin Extraction

Hydrothermal pretreatment and enzymatic saccharification of corn stover for efficient ethanol production

2013 ◽  
Vol 44 ◽  
pp. 367-372 ◽  
Author(s):  
Badal C. Saha ◽  
Tsuyoshi Yoshida ◽  
Michael A. Cotta ◽  
Kenji Sonomoto
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Enzymatic Saccharification ◽  
Hydrothermal Pretreatment
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