Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production

AbstractIonic liquid (IL) 1-ethyl-3-methylimidazolium dimethylphosphate ([Emim]DMP) was chosen as an environment-friendly solvent to enzymatically hydrolyze cellulose in situ. Under optimal reaction condition, 80.2 % of cellulose (10 mg mL−1) were converted to glucose in aqueous-IL-DMSO (φ r = 74: 25: 1) media at 55°C in 18 h. Finally, fermentability of the recovered hydrolyzates was evaluated using Saccharomyces cerevisiae which is able to ferment hydrolyzates efficiently, the ethanol production was 0.44 g g−1 of glucose within 24 h of the process. Such information is vital for the saccharification of more complex cellulose materials and for the fermentation of hydrolyzates into biofuel.

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The water consumption of sugarcane bagasse post-washing after protic ionic liquid pretreatment and its impact on 2G ethanol production

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113642 ◽

2021 ◽

Vol 169 ◽

pp. 113642

Author(s):

P.Y.S. Nakasu ◽

J.L. Ienczak ◽

S.C. Rabelo ◽

A.C. Costa

Keyword(s):

Ionic Liquid ◽

Ethanol Production ◽

Sugarcane Bagasse ◽

Water Consumption ◽

Protic Ionic Liquid ◽

Ionic Liquid Pretreatment

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Ionic Liquid 1-Butyl-3-Methylimidazolium Chloride/FeCl3 Pretreatment of Sugarcane Residue for Second Generation Bioethanol Production

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.275-277.1662 ◽

2013 ◽

Vol 275-277 ◽

pp. 1662-1665 ◽

Cited By ~ 1

Author(s):

Qiang Li ◽

Juan Juan Fei ◽

Xu Ding Gu ◽

Geng Sheng Ji ◽

Yang Liu ◽

...

Keyword(s):

Ionic Liquid ◽

Enzymatic Hydrolysis ◽

Low Temperature ◽

Second Generation ◽

Reducing Sugars ◽

Enzymatic Saccharification ◽

Cellulosic Biomass ◽

Candida Shehatae ◽

Second Generation Bioethanol ◽

Temperature Pretreatment

This study aims to establish a natural cellulosic biomass pretreatment process using ionic liquid (IL) for efficient enzymatic hydrolysis and second generation bioethanol. The IL 1-Butyl-3-methylimidazolium Chloride/FeCl3 ([Bmim]Cl/FeCl3) was selected in view of its low temperature pretreatment ability and the potential of accelerating enzymatic hydrolysis, and it could be recyclable. The yield of reducing sugars from sugarcane residue pretreated with this IL at 80 oC for 1 h reached 46.8% after being enzymatically hydrolyzed for 24 h. Sugarcane residue regenerated were hydrolyzed more easily than that treated with water. The fermentability of the hydrolyzates, obtained after enzymatic saccharification of the regenerated sugarcane residue, was transformed into bioethanol using Candida shehatae. This microbe could absorb glucose and xylose efficiently, and the ethanol production was 0.38 g/g glucose within 30 h fermentation. In conclusion, the metal ionic liquid pretreatment in low temperature shows promise as pretreatment solvent for natural biomass.

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One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase

Bioengineering ◽

10.3390/bioengineering9010017 ◽

2022 ◽

Vol 9 (1) ◽

pp. 17

Author(s):

Malinee Sriariyanun ◽

Nichaphat Kitiborwornkul ◽

Prapakorn Tantayotai ◽

Kittipong Rattanaporn ◽

Pau-Loke Show

Keyword(s):

Ionic Liquid ◽

Saline Soil ◽

Enzymatic Saccharification ◽

Value Added ◽

Specific Enzyme ◽

One Pot ◽

The One ◽

Hydrolysis Of ◽

Bacterial Cellulase ◽

Value Added Products

Ionic liquid (IL) pretreatment of lignocellulose is an efficient method for the enhancement of enzymatic saccharification. However, the remaining residues of ILs deactivate cellulase, therefore making intensive biomass washing after pretreatment necessary. This study aimed to develop the one-pot process combining IL pretreatment and enzymatic saccharification by using low-toxic choline acetate ([Ch][OAc]) and IL-tolerant bacterial cellulases. Crude cellulases produced from saline soil inhabited Bacillus sp. CBD2 and Brevibacillus sp. CBD3 were tested under the influence of 0.5–2.0 M [Ch][OAc], which showed that their activities retained at more than 95%. However, [Ch][OAc] had toxicity to CBD2 and CBD3 cultures, in which only 32.85% and 12.88% were alive at 0.5 M [Ch][OAc]. Based on the specific enzyme activities, the sugar amounts produced from one-pot processes using 1 mg of CBD2 and CBD3 were higher than that of Celluclast 1.5 L by 2.0 and 4.5 times, respectively, suggesting their potential for further application in the biorefining process of value-added products.

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