A sensitive label-free impedimetric DNA biosensor based on silsesquioxane-functionalized gold nanoparticles for Zika Virus detection

2019 ◽  
Vol 141 ◽  
pp. 111351 ◽  
Author(s):  
Marines Steinmetz ◽  
Dhésmon Lima ◽  
Adriano Gonçalves Viana ◽  
Sérgio Toshio Fujiwara ◽  
Christiana Andrade Pessôa ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Zika Virus ◽  
Virus Detection ◽  
Dna Biosensor ◽  
Label Free ◽  
Functionalized Gold Nanoparticles

scholarly journals Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Samsulida Abdul Rahman ◽  
Rafidah Saadun ◽  
Nur Ellina Azmi ◽  
Nurhayati Ariffin ◽  
Jaafar Abdullah ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Peptide Nucleic Acid ◽  
Virus Detection ◽  
Label Free ◽  
Color Changes ◽  
Phosphate Backbone ◽  
Single Base Mismatch ◽  
Target Dna ◽  
Base Mismatch

A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra. The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA.

Label-free electrochemical DNA biosensor for zika virus identification

2019 ◽  
Vol 131 ◽  
pp. 149-155 ◽  
Author(s):  
Henrique Antonio Mendonça Faria ◽  
Valtencir Zucolotto
Keyword(s):  
Zika Virus ◽  
Dna Biosensor ◽  
Label Free ◽  
Electrochemical Dna Biosensor ◽  
Virus Identification

Spectrophotometric Label-Free Determination of Lead Using Thiol-Functionalized Gold Nanoparticles

2018 ◽  
Vol 51 (8) ◽  
pp. 1208-1218
Author(s):  
Jyoti Gahlaut ◽  
Yudhishthir Singh Rajput ◽  
Sunita Meena ◽  
Dhiraj Kumar Nanda ◽  
Rajan Sharma
Keyword(s):  
Gold Nanoparticles ◽  
Label Free ◽  
Functionalized Gold Nanoparticles
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