Microsatellite markers for barnacle studies: Isolation and characterization of polymorphic microsatellite loci from the invasive barnacle Megabalanus coccopoma (Darwin, 1854) and its cross-amplification in the Southern Atlantic endemic species Megabalanus vesiculosus (Darwin, 1854)

2016 ◽  
Vol 66 ◽  
pp. 224-228 ◽  
Author(s):  
Nívia Maria Nunes Abreu ◽  
Isabela Marçal ◽  
Angela Borges Duarte ◽  
Fabio Bettini Pitombo ◽  
Anderson Vilasboa ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Endemic Species ◽  
Microsatellite Loci ◽  
Cross Amplification ◽  
Isolation And Characterization ◽  
Polymorphic Microsatellite

scholarly journals Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

HortScience ◽  
2010 ◽  
Vol 45 (4) ◽  
pp. 690-692 ◽  
Author(s):  
Jiang-Chong Wu ◽  
Jing Yang ◽  
Zhi-Jian Gu ◽  
Yan-Ping Zhang
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Moringa Oleifera ◽  
Microsatellite Primers ◽  
Genetic Studies ◽  
Isolation And Characterization ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

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