Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

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Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites

Sociobiology ◽

10.13102/sociobiology.v64i3.1692 ◽

2017 ◽

Vol 64 (3) ◽

pp. 352 ◽

Cited By ~ 1

Author(s):

Yu-Lei Dang ◽

Hong-Gui Zhang ◽

Yu-Feng Meng ◽

Min Zhang ◽

Sha Zhao ◽

...

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Population Genetic ◽

Subterranean Termites ◽

Polymorphic Markers ◽

Isolation And Characterization ◽

Expected Heterozygosity ◽

Hardy Weinberg Equilibrium ◽

Polymorphic Microsatellite

We isolated 15 and 18 highly polymorphic genomic microsatellite markers from two subterranean termites, Reticulitermes aculabialis and R. labralis, respectively. A total of 53 alleles were detected in 15 microsatellite loci of R. aculabialis, and the alleles were 3.533±1.302 (mean±SD), while the corresponding data of R. labralis were 115 detected alleles in 18 microsatellite loci with 6.389±1.754 alleles. The observed and expected heterozygosity was 0.496±0.236 and 0.564±0.125 in R. aculabialis, and 0.368±0.263 and 0.702±0.115 in R. labralis, respectively. Seven loci were highly polymorphic (PIC>0.5) in R. aculabialis, and 15 loci were highly polymorphic (PIC>0.5) in R. labralis. All loci showed Hardy–Weinberg equilibrium. These polymorphic markers provide useful tools for population genetic and breeding system studies of subterranean termites.

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Microsatellite markers for barnacle studies: Isolation and characterization of polymorphic microsatellite loci from the invasive barnacle Megabalanus coccopoma (Darwin, 1854) and its cross-amplification in the Southern Atlantic endemic species Megabalanus vesiculosus (Darwin, 1854)

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2016.04.006 ◽

2016 ◽

Vol 66 ◽

pp. 224-228 ◽

Cited By ~ 1

Author(s):

Nívia Maria Nunes Abreu ◽

Isabela Marçal ◽

Angela Borges Duarte ◽

Fabio Bettini Pitombo ◽

Anderson Vilasboa ◽

...

Keyword(s):

Microsatellite Markers ◽

Endemic Species ◽

Microsatellite Loci ◽

Cross Amplification ◽

Isolation And Characterization ◽

Polymorphic Microsatellite

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De novo development and characterization of polymorphic microsatellite markers in a schilbid catfish, Silonia silondia (Hamilton, 1822) and their validation for population genetic studies

Molecular Biology Reports ◽

10.1007/s11033-016-3941-y ◽

2016 ◽

Vol 43 (2) ◽

pp. 91-98 ◽

Cited By ~ 4

Author(s):

Sangeeta Mandal ◽

J. K. Jena ◽

Rajeev K. Singh ◽

Vindhya Mohindra ◽

W. S. Lakra ◽

...

Keyword(s):

Microsatellite Markers ◽

Population Genetic ◽

De Novo ◽

Genetic Studies ◽

Polymorphic Microsatellite

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Isolation and Characterization of 17 Polymorphic Microsatellite Loci for Musella lasiocarpa (Musaceae)

HortScience ◽

10.21273/hortsci.44.7.2041 ◽

2009 ◽

Vol 44 (7) ◽

pp. 2041-2042 ◽

Cited By ~ 2

Author(s):

Chun-yan Yang ◽

Yuan Huang ◽

Chunlin Long

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Southwest China ◽

Microsatellite Primers ◽

Genetic Population ◽

Allele Number ◽

Isolation And Characterization ◽

Hardy Weinberg Equilibrium ◽

Polymorphic Microsatellite

Seventeen microsatellite markers were isolated from 24 wild individuals collected from three populations of Musella lasiocarpa (Franch.) C. Y. Wu ex H. W. Li, a plant endemic to southwest China. The average allele number of these markers was 3.6 per locus, ranging from two to seven. The expected (He) and observed (Ho) heterozygosities varied from 0.4672 to 0.8183 and from 0.0417 to 1, respectively. Eleven loci were significantly deviated from Hardy-Weinberg equilibrium as a result of the heterozygote deficiency. These microsatellite primers will be used for studying genetic population structure and genetic diversity of M. lasiocarpa.

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Isolation and characterization of 10 polymorphic microsatellite loci in the rarest European damselfly, Coenagrion hylas (Odonata: Coenagrionidae)

International Journal of Odonatology ◽

10.23797/2159-6719_24_17 ◽

2021 ◽

Vol 24 ◽

pp. 227-232

Author(s):

M. Landmann ◽

M. Schilling ◽

A. Landmann ◽

F. M. Steiner ◽

B. C. Schlick-Steiner

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Population Connectivity ◽

Vulnerable Species ◽

Central European ◽

Limited Distribution ◽

Isolation And Characterization ◽

Polymorphic Microsatellite ◽

European Populations

Within Europe, the damselfly Coenagrion hylas has a very limited distribution and is regarded as a vulnerable species. For studying migration and population connectivity in the Central European populations, 10 microsatellite markers were developed for this species. The loci were screened on 24 individuals collected at Lech valley, Tyrol, Austria. The values for expected and observed heterozygosity ranged from 0.192 to 0.802 and from 0.208 to 0.917, respectively. All developed markers were polymorphic.

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