Partial replacement of fish meal with Moringa oleifera leaf meal in practical diets of Cirrhinus mrigala fingerlings

Fish protein is serving as a source of nutrition for protein starving world. However, sustainable aquaculture products require inexpensive plant by-products due to finite sources of fish meal. Therefore, this study was conducted to examine nutrient utilization, growth performance and hematological indices of Cirrhinus mrigala fingerlings fed on Moringa oleifera leaf meal (MOLM) based diets. Fish were fed with six isonitrogenous and isoenergetic diets having MOLM as a substitute of fish meal (FM) at the levels of 0%, 10%, 20%, 30%, 40% and 50% for the period of 90 days. Fingerlings having initial weight 6.35±0.04g were reared in triplicate tanks at the stocking density of 15 and hand fed at the rate of 5% of total biomass twice regularly. Chromic oxide inclusion level was 1% in diets. After analysis, maximum growth performance and improved digestibility of nutrients were found in fish fed with diet at 10% replacement level as compared to fish fed on control diet and other test diets. Additionally, it was found that the red blood cells, white blood cells, hemoglobin and mean corpuscular hemoglobin concentration of fish showed a significantly (p<0.05) inverse correlation with the increase in MOLM. In present research, it was concluded that MOLM has good potential to be used as a FM substitute in C. mrigala diet with maximum effect at 10% showing positive hematological indices.

Amelioration potential of Moringa oleifera extracts against sodium arsenate induced embryotoxicity and genotoxicity in mouse (Mus musculus)

Previous studies have suggested that arsenic crosses the placenta and affects the fetus development. The study under consideration aims to show comparative ameliorative effect of Moringa oleifera leaf and flower extracts against sodium arsenate induced fetus toxicity of mice. Pregnant mice (N=44) were kept in lab and divided into eleven group from (A to K) and were orally administered the doses 6 mg/kg, 12 mg/kg for sodium arsenate, 150 mg/kg and 300 mg/kg for Moringa oleifera leaf extracts (MOLE) and 150 mg/kg and 300 mg/kg for Moringa oleifera flower extracts (MOFE) comparing with control. The investigation revealed evident reduction in the fetuses weight, hind limb, fore limb, tail and snout length, crown rump and head circumferences well as malformations in tail, feet, arms, legs, skin and eyes in the negative control group (only administered with sodium arsenate). Co-administration of sodium arsenate with MOLE and MOFE ameliorate the reversed effect of sodium arsenate on the shape, length, body weight and DNA damage of fetus significantly at 95% confidence interval. However, Moringa oleifera leaf extract showed more significant results in comparison to Moringa oleifera flower extract. Hence concluded that Moringa oleifera leaf extract ameliorated the embryo toxic effects of sodium arsenate and can be used against environmental teratogens.

Potential of Moringa Oleifera Seed as a Natural Adsorbent for Wastewater Treatment

The present study deals with the appropriateness of the coagulation process using natural coagulant Moringa oleifera seed. Natural coagulants are useful for the treatment of wastewater because of its sustainability, cost-effectiveness, non-toxicity and lesser quantity of sludge formation. M. oleifera seed having a chemical composition of polypeptides having 6 amino acids like arginine acid, methionine acid, glutamic acid, phenylalanine, threonine, and histidine. M. oleifera is also known as a cationic polyelectrolyte and having molecular weight 6,000 to16,000 Dalton. The main objective of research work is the application of the M. oleifera seed as a natural adsorbent to treat synthetic dairy wastewater. The effects of pH, agitation time, the dose of sorbent and efficacy of M. oleifera seeds kernel for turbidity removal was assessed. M. oleifera seed eliminates turbidity 95 % and colour 94 % using 0.22 gm pod powder, and 0.2 L of 1.0 g/L synthetic dairy wastewater. Naturally dried M. oleifera seeds remove turbidity 95 %, sundried seeds remove turbidity 52 % and oven-dried seeds 45 %. As naturally dried M. oleifera pod having more surface area for adsorption and inter-particulate bridging which extract the extra active ingredients. pH range between 5 and 8 is more suitable to degrade the turbidity and colour. It is concluded that in the presence of an aqueous soluble cationic coagulant protein has great potential to remove the turbidity and colour of wastewater. HIGHLIGHTS oleifera seed having a chemical composition of polypeptides having 6 amino acids like arginine acid, methionine acid, glutamic acid, phenylalanine, threonine, and histidine oleifera seeds consist of crude fiber, lignin, hemicellulose, and cellulose. It also contains amino functional groups (R-NH3), carboxyl group (C=O), and fiber carbonaceous. The functional group present in M. oleifera seeds is dissociated during the adsorption process at various pH oleifera has good property of coagulation-flocculation (C-F) The effectiveness of naturally dried seed kernel is more effective than other seed kernels GRAPHICAL ABSTRACT

Effect of Cytokinins on Micropropagation of Moringa Oleifera Lam. and Genomic Stability Assessment Using Flow-Cytometry

Moringa oleifera Lam. is a multipurpose medicinal plant of the family Moringaceae which has been widely utilized as a pharmaceutical remedy to treat a wide range of diseases. In addition, the tree has several applications in human nutrition as well as livestock feeding. M. oleifera is easily multiplied through epigeal germination (recalcitrant) but seed propagated plants are heterogeneous and take longer to reach fruit-bearing age. As an alternative, branch cuttings have been used but their establishment is erratic and often leads to reduced growth of the mother plant. Thus, to produce superior planting materials, in-vitro propagation has become paramount. As a result, several studies using a limited range of cytokinin have been undertaken to multiply M. oleifera through tissue culture. Otherwise, a study was conducted to examine the effect of five different cytokinins on in-vitro regeneration of this tree species. Results showed that nodal explants cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 6-Benzylaminopurine (BAP) and subsequently rooted on basic MS media was the most optimal treatment. Furthermore, acclimatization of plantlets in sterile soil substrate and perlite (1:3;v/v) under transparent polythene sheet for 7 days resulted in survival rate of 100%. Assessment of genetic fidelity using flow cytometry revealed that surface sterilization alongside cytokinin treatments produced plantlets that were genetically stable regardless of the growth regulator used. Thus, the in-vitro protocol developed in this study can be utilized for in-vitro studies and mass propagation of this imperative plant species.

Antiplaque Efficacy of a Novel Moringa oleifera Dentifrice: A Randomized Clinical Crossover Study

Objectives The use of herbal dentifrices has grown exponentially over the years. They are categorically referred to as ethnomedicines. Various agents have been tried with contradicting findings based on phytopharmacological analysis. Miswak is one agent which has been used over the years. A novel Moringa oleifera-based dentifrice has shown promising results in terms of its cytotoxicity, biocompatibility, and as a potent anti-inflammatory agent. Therefore, the present study aims to compare the efficacy of two commercially available miswak- and moringa-based herbal dentifrices on the reduction of plaque and gingivitis scores. Materials and Methods This randomized clinical crossover study included 20 subjects with mild to moderate gingivitis. The study was conducted over a total examination period of 20 days with a wash-out period of 2 weeks between the use of both the toothpastes. The plaque index and gingival index of the study subjects were recorded at the designated time intervals throughout the study period. Statistical Analysis The data collected were entered on Microsoft Excel, and statistical analysis using SPSS software (SPSS version 28, IBM Corp, Armonk, New York, United States) was done. The statistical test used was the Wilcoxon signed-rank test. Moreover, p ≤0.05 was considered significant. Results The results showed that the reduction in mean gingival index scores from baseline to day 3 was more statistically significant in the moringa-based dentifrice. Similarly, the plaque index scores showed statistically significant reduction following the use of the moringa-based dentifrice when compared with the miswak dentifrice. This study reveals that the moringa dentifrice is a safe and effective agent in reducing plaque accumulation and treating gingival inflammation. Conclusion The current study aims to provide an insight into the possible role of moringa dentifrice as a possible adjunctive oral hygiene aid.

CRESCIMENTO VEGETATIVO DA MORINGA EM DISTINTOS REGIMES DE IRRIGAÇÃO ASSOCIADOS A COMPOSIÇÕES DE DIFERENTES SUBSTRATOS

CRESCIMENTO VEGETATIVO DA MORINGA EM DISTINTOS REGIMES DE IRRIGAÇÃO ASSOCIADOS A COMPOSIÇÕES DE DIFERENTES SUBSTRATOS VALDIR MOURA DE OLIVEIRA JUNIOR1; THAYS SOUSA LOPES1; JOÃO VALDENOR PEREIRA FILHO1; JAILDO RIBEIRO BARBOSA1; ROBERT WILLIAM FERREIRA SOARES1; CARMEM CRISTINA MARECO DE SOUSA PEREIRA2 1Centro Integrado de Ensino Superior, Universidade Estadual do Piauí (UESPI), Rua Almir Benvindo, s/n, bairro Aeroporto, 64860-000, Uruçuí, Piauí, Brasil. [email protected]; [email protected]; [email protected]; [email protected]; [email protected] 2 Departamento de Engenharia Agrícola, Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manuel de Medeiros, s/n - Dois Irmãos, 52171-900, Recife, Pernambuco, Brasil. [email protected] 1 RESUMO A moringa possui ampla adaptabilidade e se condiciona, com muita facilidade, ao clima e solo do Nordeste brasileiro. Ainda são escassas as informações sobre a produção de mudas desta espécie sob composições de substratos associados a regimes de irrigação. Desta forma, o objetivo desse trabalho foi caracterizar o comportamento vegetativo de plantas de moringa submetidas a distintas composições de substratos e regimes de irrigação. O experimento foi realizado em ambiente telado, no período de setembro a outubro de 2020, na área experimental da Universidade Estadual do Piauí, Uruçuí. Adotou-se um delineamento experimental em esquema fatorial, sendo, o primeiro fator, dois regimes de irrigação (50 e 100% da ETo) e o segundo fator, cinco substratos (SB1 = latossolo vermelho; SB2 = substrato comercial; SB3 = solo + esterco; SB4 = solo + cinza vegetal; SB5 = solo + borra de café), com 5 repetições. Aos 45 dias após a semeadura (DAS) foram avaliadas a altura de plantas e o diâmetro do caule. Os parâmetros de crescimento inicial da cultura da moringa foram afetados significativamente pela interação (regimes de irrigação x substratos), sendo os melhores resultados obtidos, na maioria dos substratos utilizados, com a aplicação do regime hídrico de 100% da ETo. Palavras-chave: Moringa oleifera Lam, produção de mudas, déficit hídrico. OLIVEIRA JUNIOR, V. M.; LOPES, T. S.; PEREIRA FILHO, J. V.; BARBOSA, J. R.; SOARES, R. W. F.; PEREIRA, C. C. M. S. VEGETATION GROWTH OF MORINGA IN DIFFERENT IRRIGATION REGIMES ASSOCIATED WITH COMPOSITIONS OF DIFFERENT SUBSTRATES 2 ABSTRACT Moringa has wide adaptability and is very easily conditioned to the climate and soil of northeastern Brazil. Information on the production of seedlings of this species under compositions of substrates associated with irrigation regimes is still scarce. Thus, this work aimed to characterize the vegetative behavior of moringa plants submitted to different compositions of substrates and irrigation regimes. The experiment was carried out in a screened environment, from September to October 2020, in the experimental area of ​​the State University of Piauí, Uruçuí. An experimental design was adopted in a factorial scheme, the first factor being two irrigation regimes (50 and 100% of ETo) and the second factor, five substrates (SB1 = red oxisol; SB2 = commercial substrate; SB3 = soil + manure; SB4 = soil + vegetable ash; SB5 = soil + coffee grounds), with 5 repetitions. At 45 days after sowing (DAS), plant height and stem diameter was evaluated. The initial growth parameters of the moringa crop were significantly affected by the interaction (irrigation regimes x substrates), with the best results obtained, in most of the substrates used, with the application of the water regime of 100% of ETo. Keywords: Moringa oleifera Lam, seedling production, water deficit.