A novel slow-tight binding serine protease inhibitor from eastern oyster (Crassostrea virginica) plasma inhibits perkinsin, the major extracellular protease of the oyster protozoan parasite Perkinsus marinus

2006 ◽  
Vol 145 (1) ◽  
pp. 16-26 ◽  
Author(s):  
Qing-Gang Xue ◽  
Grover L. Waldrop ◽  
Kevin L. Schey ◽  
Naoki Itoh ◽  
Masahiro Ogawa ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Serine Protease ◽  
Crassostrea Virginica ◽  
Tight Binding ◽  
Protozoan Parasite ◽  
Eastern Oyster ◽  
Serine Protease Inhibitor ◽  
Extracellular Protease ◽  
Perkinsus Marinus

scholarly journals Perkinsus marinus Extracellular Protease Modulates Survival of Vibrio vulnificus in Eastern Oyster (Crassostrea virginica) Hemocytes

1999 ◽  
Vol 65 (9) ◽  
pp. 4261-4263 ◽  
Author(s):  
B. D. Tall ◽  
J. F. La Peyre ◽  
J. W. Bier ◽  
M. D. Miliotis ◽  
D. E. Hanes ◽  
...  
Keyword(s):  
Serine Protease ◽  
Crassostrea Virginica ◽  
Time Course ◽  
Vibrio Vulnificus ◽  
Eastern Oyster ◽  
Intracellular Survival ◽  
Extracellular Protease ◽  
Perkinsus Marinus ◽  
In Vitro Effects

ABSTRACT The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificusin oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced byP. marinus suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in oyster tissues.

Sign in / Sign up

Export Citation Format

Share Document