Identification and characterization of a new germline-specific marker vasa gene and its promoter in the giant freshwater prawn Macrobrachium rosenbergii

Author(s):  
Xue-Ying He ◽  
Xiang Fang ◽  
Bi-Yun Luo ◽  
Gao-Feng Qiu
2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Hirak Kumar Barman ◽  
Swagat Kumar Patra ◽  
Varsha Das ◽  
Shibani Dutta Mohapatra ◽  
Pallipuram Jayasankar ◽  
...  

The giant freshwater prawn,Macrobrachium rosenbergii,is an economically important species. It is a euryhaline shrimp, surviving in wide-range salinity conditions. A change in gene expression has been suggested as an important component for stress management. To better understand the osmoregulatory mechanisms mediated by the gill, a subtractive and suppressive hybridization (SSH) tool was used to identify expressed transcripts linked to adaptations in saline water. A total of 117 transcripts represented potentially expressed under salinity conditions. BLAST analysis identified 22% as known genes, 9% as uncharacterized showing homologous to unannotated ESTs, and 69% as unknown sequences. All the identified known genes representing broad spectrum of biological pathways were particularly linked to stress tolerance including salinity tolerance. Expression analysis of 10 known genes and 7 unknown/uncharacterized genes suggested their upregulation in the gills of prawn exposed to saline water as compared to control indicating that these are likely to be associated with salinity acclimation. Rapid amplification of cDNA ends (RACE) was used for obtaining full-length cDNA of MRSW-40 clone that was highly upregulated during salt exposure. The sequenced ESTs presented here will have potential implications for future understanding about salinity acclimation and/or tolerance of the prawn.


Aquaculture ◽  
2019 ◽  
Vol 507 ◽  
pp. 56-68 ◽  
Author(s):  
Tipsuda Thongbuakaew ◽  
Saowaros Suwansa-ard ◽  
Prapee Sretarugsa ◽  
Prasert Sobhon ◽  
Scott F. Cummins

Heliyon ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. e05898
Author(s):  
Tipsuda Thongbuakaew ◽  
Chanudporn Sumpownon ◽  
Attakorn Engsusophon ◽  
Napamanee Kornthong ◽  
Charoonroj Chotwiwatthanakun ◽  
...  

2008 ◽  
Vol 10 (5) ◽  
pp. 1473-1475 ◽  
Author(s):  
Shripathi Bhat ◽  
Alok Patel ◽  
Paramananda Das ◽  
Prem K. Meher ◽  
Bidu R. Pillai ◽  
...  

2014 ◽  
Vol 44 (2) ◽  
pp. 359-369 ◽  
Author(s):  
Xiao-Wen Zhang ◽  
Xian-Wei Wang ◽  
Ying Huang ◽  
Kai-Min Hui ◽  
Yan-Ru Shi ◽  
...  

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