Genotoxicity assessment of pulp and paper mill effluent before and after bacterial degradation using Allium cepa test

Chemosphere ◽  
2017 ◽  
Vol 169 ◽  
pp. 642-650 ◽  
Author(s):  
Izharul Haq ◽  
Sharad Kumar ◽  
Abhay Raj ◽  
Mohtashim Lohani ◽  
G.N.V. Satyanarayana
2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Izharul Haq ◽  
Vineeta Kumari ◽  
Sharad Kumar ◽  
Abhay Raj ◽  
Mohtashim Lohani ◽  
...  

Pulp and paper mill effluent induced phytotoxicity and genotoxicity in mung bean (Vigna radiata L.) and root tip cells of onion (Allium cepa L.) were investigated. Physicochemical characteristics such as electrical conductivity (EC), biological oxygen demand (BOD5), chemical oxygen demand (COD), and total phenols of the pulp and paper mill effluent were beyond the permissible limit specified for the discharge of effluent in inland water bodies. Compared to control plants, seedling exposed to 100% effluent concentration showed a reduction in root and shoot length and biomass by 65%, 67%, and 84%, respectively, after 5 days of treatment. A. cepa root tip cells exposed to effluent concentrations ranging from 25 to 100% v/v showed a significant decrease in mitotic index (MI) from 32 to 11% with respect to control root tip cells (69%) indicating effluent induced cytotoxicity. Further, the effluent induced DNA damage as evidenced by the presence of various chromosomal aberrations like stickiness, chromosome loss, anaphase bridge, c-mitosis, tripolar anaphase, vagrant chromosome, and telophase bridge and micronucleated and binucleated cell in A. cepa. Findings of the present study indicate that pulp and paper mill effluents may act as genotoxic and phytotoxic agents in plant model system.


1997 ◽  
Vol 35 (2-3) ◽  
pp. 205-212 ◽  
Author(s):  
H. T. Adisesha ◽  
S. Purwati ◽  
P. R. Panggabean ◽  
S. E. Sarief

Padalarang pulp and paper mill, a soda pulp and paper mill without chemical recovery, located near Bandung, Indonesia, discharges untreated effluent mixed with domestic waste water into surrounding rice fields. For more than 60 years, paddy has been harvested 3 times a year. An in-depth study to characterize the effluent, the soil and the biomass production was conducted to define the evidence. A field study using four experimental plots indicated that soil irrigated with effluent had a higher concentration of potassium, calcium, sodium ions and greater cation exchange capacity than near-by soil. Analysis of well water showed that the effluent had no effect on the ground water. A reduction of suspended solids and BOD occurred in the effluent while flowing through rice fields. The yields of paddy demonstrated significant positive effect of effluent either in dry or in rainy season, while zeolites as soil amendment did not give significant effect to the already high paddy crop yield.


1994 ◽  
Vol 72 (2) ◽  
pp. 247-251 ◽  
Author(s):  
R. A. Khan ◽  
D. E. Barker ◽  
K. Williams-Ryan ◽  
R. G. Hooper

Samples of longhorn sculpin (Myoxocephalus octodecemspinosus) were exposed to sediment contaminated with crude oil or pulp and paper mill effluent for periods up to 13 months in the laboratory. Other samples were collected at sites where crude oil or effluent from a pulp and paper mill are discharged. The intensity of gill infections of Trichodina spp. on exposed fish was significantly higher than on controls 5, 9, and 13 months after exposure. The intensity of the ciliates was also greater on sculpins collected near an oil-receiving terminal than on those sampled 5 km from the polluted site. Field collections of longhorn and shorthorn (Myoxocephalus scorpius) sculpins at and distant from a pulp and paper mill had high and low intensities of the ciliates, respectively. Similarly, the intensity of trichodinid ciliates was also significantly greater in longhorn sculpins exposed to effluent-contaminated sediment than in controls 5 months after exposure. The results suggest that the intensity of gill-inhibiting species such as trichodinids in susceptible fish hosts increases after chronic exposure to crude oil and to pulp and paper mill effluent, and the parasites may serve as indicators of pollution.


2011 ◽  
Vol 13 (3) ◽  
pp. 37-41
Author(s):  
T. Kannadasan ◽  
V. Sivakumar ◽  
C. Basha ◽  
Arun Parwate ◽  
K. Senthilkumar ◽  
...  

COD reduction studies of paper mill effluent using a batch recirculation electrochemical method The conventional method of treating pulp and paper mill effluent involves the biological oxidation by bacterial action of aerobic and anaerobic conditions and aerobic lagooning method, which are less efficiency of removing COD. To overcome the drawbacks of the existing treatment process, in the present work an attempt has been made to study the electro oxidative destruction of the pulp and paper mill effluent using an electrochemical method and the effect of various parameters such as concentration of supporting electrolytes, current densities, flow rates of electrolyte and reservoir volumes of the effluent were conducted. From the experimental results it is observed that the rate of reduction of COD of the effluent increased with an increase in the supporting electrolyte (sodium chloride) concentration, current density where as it decreased with increase in the reservoir volume and the flow rate of electrolyte. The residence time distributions studies have also been conducted to study the behavior of the electrochemical reactor.


2007 ◽  
Vol 7 (15) ◽  
pp. 2164-2167 ◽  
Author(s):  
A.Y. Zahrim ◽  
Melissa L. Gilbert . ◽  
Jidon Janaun .

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